Difference between revisions of "Zwf"

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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
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=== Extended information on the protein ===
 
=== Extended information on the protein ===
  
* '''Kinetic information:''' Two Substrate Michaelis-Menten [http://www.ncbi.nlm.nih.gov/pubmed/6292660 PubMed]
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* '''Kinetic information:'''  
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** Two Substrate Michaelis-Menten [http://www.ncbi.nlm.nih.gov/pubmed/6292660 PubMed]
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** specific activity: 0.375 µmol min<sup>-1</sup> (mg protein)<sup>-1</sup> {{PubMed|24571712}}
  
* '''Domains:'''  
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* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
* '''Cofactor(s):''' Mg2+, Mn2+, Ca2+
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* '''[[Cofactors]]:''' Mg2+, Mn2+, Ca2+
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
 
** NAD+, NADP+ and NADPH affect the enzyme kinetic [http://www.ncbi.nlm.nih.gov/pubmed/6292660 PubMed]
 
** NAD+, NADP+ and NADPH affect the enzyme kinetic [http://www.ncbi.nlm.nih.gov/pubmed/6292660 PubMed]
  
* '''Interactions:'''
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* '''[[SubtInteract|Interactions]]:'''
  
* '''Localization:'''
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* '''[[Localization]]:'''  
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=zwf_2479156_2480625_1 zwf] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=zwf_2479156_2480625_1 zwf] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
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* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:''' constitutive [http://www.ncbi.nlm.nih.gov/sites/entrez/12850135 PubMed]
 
* '''Regulation:''' constitutive [http://www.ncbi.nlm.nih.gov/sites/entrez/12850135 PubMed]
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=References=
 
=References=
  
<pubmed>6292660,12850135,19779711,7881907 </pubmed>
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<pubmed>6292660,12850135,19779711,7881907 24571712</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 16:32, 28 February 2014

  • Description: glucose 6-phosphate dehydrogenase, pentose-phosphate pathway

Gene name zwf
Synonyms yqjJ
Essential no
Product glucose 6-phosphate dehydrogenase
Function initiation of the pentose phosphate pathway
Gene expression levels in SubtiExpress: zwf
Metabolic function and regulation of this protein in SubtiPathways:
zwf
MW, pI 55 kDa, 5.28
Gene length, protein length 1467 bp, 489 aa
Immediate neighbours rnz, gndA
Sequences Protein DNA DNA_with_flanks
Genetic context
Zwf context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Zwf expression.png















Categories containing this gene/protein

carbon core metabolism

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU23850

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: D-glucose 6-phosphate + NADP+ = D-glucono-1,5-lactone 6-phosphate + NADPH (according to Swiss-Prot)
  • Protein family: glucose-6-phosphate dehydrogenase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
    • Two Substrate Michaelis-Menten PubMed
    • specific activity: 0.375 µmol min-1 (mg protein)-1 PubMed
  • Modification:
  • Effectors of protein activity:
    • NAD+, NADP+ and NADPH affect the enzyme kinetic PubMed

Database entries

  • Structure: 1DPG (from Leuconostoc Mesenteroides, 45% identity, 62% similarity) PubMed
  • KEGG entry: [3]

Additional information

The enzyme is a dimer PubMed

Expression and regulation

  • Regulation: constitutive PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:
  • FLAG-tag construct: GP1401 (spc, based on pGP1331), available in the Stülke lab

Labs working on this gene/protein

Your additional remarks

References