Difference between revisions of "CwlT"
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| − | * '''Description:''' cell wall hydrolase, C-terminal domain hydrolyzes bond between D-Glu and m-DAP <br/><br/> | + | * '''Description:''' cell wall hydrolase, required for conjugation of ICE''Bs1'', C-terminal domain hydrolyzes bond between D-Glu and m-DAP <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 12: | Line 12: | ||
|style="background:#ABCDEF;" align="center"| '''Product''' || cell wall hydrolase | |style="background:#ABCDEF;" align="center"| '''Product''' || cell wall hydrolase | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || conjugation of ICE''Bs1'' |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU04970 cwlT] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU04970 cwlT] | ||
| Line 64: | Line 64: | ||
* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
| − | + | ** hydrolysis of peptidoglycan (linkage between N-acetylmuramic acid and N-acetylglucosamine and bond between D-γ-glutamate and meso-diaminopimelic acid) {{PubMed|18305117}} | |
* '''Protein family:''' nlpC/p60 family (according to Swiss-Prot) | * '''Protein family:''' nlpC/p60 family (according to Swiss-Prot) | ||
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* '''[[Domains]]:''' | * '''[[Domains]]:''' | ||
| − | ** N-terminal domain is an N-acetylmuramidase that cleaves the linkage between N-acetylmuramic acid and N-acetylglucosamine {{PubMed| | + | ** N-terminal domain is an N-acetylmuramidase that cleaves the linkage between N-acetylmuramic acid and N-acetylglucosamine {{PubMed|18305117}} |
| − | ** C-terminal endopeptidase domain cleaves the bond between D-γ-glutamate and meso-diaminopimelic acid | + | ** C-terminal endopeptidase domain cleaves the bond between D-γ-glutamate and meso-diaminopimelic acid {{PubMed|18305117}} |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| Line 86: | Line 86: | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
| + | ** secreted (with signal peptide) {{PubMed|24532767}} | ||
| + | ** may be a lipoprotein, but the lipid anchor is not required for function {{PubMed|24532767}} | ||
=== Database entries === | === Database entries === | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yddH_544022_545011_1 cwlT] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yddH_544022_545011_1 cwlT] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
Revision as of 18:17, 18 February 2014
- Description: cell wall hydrolase, required for conjugation of ICEBs1, C-terminal domain hydrolyzes bond between D-Glu and m-DAP
| Gene name | cwlT |
| Synonyms | yddH |
| Essential | |
| Product | cell wall hydrolase |
| Function | conjugation of ICEBs1 |
| Gene expression levels in SubtiExpress: cwlT | |
| MW, pI | 36 kDa, 8.632 |
| Gene length, protein length | 987 bp, 329 aa |
| Immediate neighbours | yddG, yddI |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
cell wall degradation/ turnover
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU04970
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- hydrolysis of peptidoglycan (linkage between N-acetylmuramic acid and N-acetylglucosamine and bond between D-γ-glutamate and meso-diaminopimelic acid) PubMed
- Protein family: nlpC/p60 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P96645
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- induced by mitomycin C PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Tyler DeWitt, Alan D Grossman
The bifunctional cell wall hydrolase CwlT is needed for conjugation of the integrative and conjugative element ICEBs1 in Bacillus subtilis and B. anthracis.
J Bacteriol: 2014, 196(8);1588-96
[PubMed:24532767]
[WorldCat.org]
[DOI]
(I p)
Tatsuya Fukushima, Toshihiko Kitajima, Hiroyuki Yamaguchi, Qin Ouyang, Kazumi Furuhata, Hiroki Yamamoto, Toshio Shida, Junichi Sekiguchi
Identification and characterization of novel cell wall hydrolase CwlT: a two-domain autolysin exhibiting n-acetylmuramidase and DL-endopeptidase activities.
J Biol Chem: 2008, 283(17);11117-25
[PubMed:18305117]
[WorldCat.org]
[DOI]
(P p)
