Difference between revisions of "NadF"
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= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
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=The gene= | =The gene= | ||
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=== Additional information=== | === Additional information=== | ||
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=The protein= | =The protein= | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' allosterically activated by quinolinic acid | * '''Effectors of protein activity:''' allosterically activated by quinolinic acid | ||
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[yjbL]]-[[ | + | * '''Operon:''' ''[[yjbL]]-[[sasB]]-[[nadF]]-[[yjbO]]'' {{PubMed|18067544}} |
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ppnKA_1237660_1238460_1 nadF] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ppnKA_1237660_1238460_1 nadF] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' expressed during exponential growth | * '''Regulation:''' expressed during exponential growth | ||
Revision as of 16:01, 6 February 2014
- Description: NAD kinase
| Gene name | nadF |
| Synonyms | yjbN, ppnK |
| Essential | yes PubMed |
| Product | inorganic polyphosphate/ATP-NAD kinase |
| Function | generation of NADP from NAD |
| Gene expression levels in SubtiExpress: nadF | |
| MW, pI | 29 kDa, 5.991 |
| Gene length, protein length | 798 bp, 266 aa |
| Immediate neighbours | yjbM, yjbO |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
biosynthesis of cofactors, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU11610
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + NAD+ = ADP + NADP+ (according to Swiss-Prot)
- Protein family: NAD kinase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity: allosterically activated by quinolinic acid
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O31612
- KEGG entry: [2]
- E.C. number: 2.7.1.23
Additional information
Expression and regulation
- Regulation: expressed during exponential growth
- Regulatory mechanism:
- Additional information:
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Hideaki Nanamiya, Koji Kasai, Akira Nozawa, Choong-Soo Yun, Takakuni Narisawa, Kana Murakami, Yousuke Natori, Fujio Kawamura, Yuzuru Tozawa
Identification and functional analysis of novel (p)ppGpp synthetase genes in Bacillus subtilis.
Mol Microbiol: 2008, 67(2);291-304
[PubMed:18067544]
[WorldCat.org]
[DOI]
(P p)
Silvia Garavaglia, Alessandro Galizzi, Menico Rizzi
Allosteric regulation of Bacillus subtilis NAD kinase by quinolinic acid.
J Bacteriol: 2003, 185(16);4844-50
[PubMed:12897004]
[WorldCat.org]
[DOI]
(P p)
