Difference between revisions of "BioF"

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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU30220 bioF]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU30220 bioF]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/biotin/index.html Biotin]'''
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=bioF bioF]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 6.285   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 6.285   

Revision as of 11:29, 7 January 2014

  • Description: 8-amino-7-oxononanoate synthase

Gene name bioF
Synonyms
Essential no
Product 8-amino-7-oxononanoate synthase
Function biosynthesis of biotin
Gene expression levels in SubtiExpress: bioF
Metabolic function and regulation of this protein in SubtiPathways:
bioF
MW, pI 42 kDa, 6.285
Gene length, protein length 1167 bp, 389 aa
Immediate neighbours bioD, bioA
Sequences Protein DNA DNA_with_flanks
Genetic context
BioF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BioF expression.png
























Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

BirA regulon

The gene

Basic information

  • Locus tag: BSU30220

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 6-carboxyhexanoyl-CoA + L-alanine = 8-amino-7-oxononanoate + CoA + CO2 (according to Swiss-Prot)
  • Protein family: class-II pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed in the presence of biotin (BirA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews: PubMed

Original publications