Difference between revisions of "Zwf"
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23850 zwf] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23850 zwf] | ||
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− | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/ | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=zwf zwf]''' |
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 55 kDa, 5.28 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 55 kDa, 5.28 |
Revision as of 11:09, 7 January 2014
- Description: glucose 6-phosphate dehydrogenase, pentose-phosphate pathway
Gene name | zwf |
Synonyms | yqjJ |
Essential | no |
Product | glucose 6-phosphate dehydrogenase |
Function | initiation of the pentose phosphate pathway |
Gene expression levels in SubtiExpress: zwf | |
Metabolic function and regulation of this protein in SubtiPathways: zwf | |
MW, pI | 55 kDa, 5.28 |
Gene length, protein length | 1467 bp, 489 aa |
Immediate neighbours | rnz, gndA |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU23850
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: D-glucose 6-phosphate + NADP+ = D-glucono-1,5-lactone 6-phosphate + NADPH (according to Swiss-Prot)
- Protein family: glucose-6-phosphate dehydrogenase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information: Two Substrate Michaelis-Menten PubMed
- Domains:
- Modification:
- Cofactor(s): Mg2+, Mn2+, Ca2+
- Effectors of protein activity:
- NAD+, NADP+ and NADPH affect the enzyme kinetic PubMed
- Interactions:
- Localization:
Database entries
- UniProt: P54547
- KEGG entry: [3]
- E.C. number: 1.1.1.49
Additional information
The enzyme is a dimer PubMed
Expression and regulation
- Operon: zwf PubMed
- Sigma factor:
- Regulation: constitutive PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- SM-NB3 (zwf-spc), available in Anne Galinier's and Boris Görke's labs
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Yun Xia Duan, Tao Chen, Xun Chen, Xue Ming Zhao
Overexpression of glucose-6-phosphate dehydrogenase enhances riboflavin production in Bacillus subtilis.
Appl Microbiol Biotechnol: 2010, 85(6);1907-14
[PubMed:19779711]
[WorldCat.org]
[DOI]
(I p)
Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135]
[WorldCat.org]
[DOI]
(P p)
P Rowland, A K Basak, S Gover, H R Levy, M J Adams
The three-dimensional structure of glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides refined at 2.0 A resolution.
Structure: 1994, 2(11);1073-87
[PubMed:7881907]
[WorldCat.org]
[DOI]
(P p)
S Ujita, K Kimura
Glucose-6-phosphate dehydrogenase, vegetative and spore Bacillus subtilis.
Methods Enzymol: 1982, 89 Pt D;258-61
[PubMed:6292660]
[WorldCat.org]
[DOI]
(P p)