DapB

• Description: dihydrodipicolinate reductase (NADPH)
  
  

Gene name	dapB
Synonyms
Essential	yes PubMed
Product	dihydrodipicolinate reductase (NADPH)
Function	biosynthesis of lysine and peptidoglycan
Gene expression levels in SubtiExpress: dapB
Metabolic function and regulation of this protein in SubtiPathways: dapB
MW, pI	29 kDa, 5.057
Gene length, protein length	801 bp, 267 aa
Immediate neighbours	mgsA, ypjD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis/ acquisition of amino acids, Biosynthesis of cell wall components, essential genes

This gene is a member of the following regulons

Spx regulon

The gene

Basic information

• Locus tag: BSU22490

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2,3,4,5-tetrahydrodipicolinate + NADP⁺ = 2,3-dihydrodipicolinate + NADPH (according to Swiss-Prot)

• Protein family: glucose-6-phosphate dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors: NADP⁺

• Effectors of protein activity:

• Interactions:
  • forms tetramers PubMed

• Localization:

Database entries

• Structure: 1YL6 (from Mycobacterium tuberculosis, 46% identity, 67% similarity) PubMed

• UniProt: P42976

• KEGG entry: [2]

• E.C. number: 1.3.1.26

Expression and regulation

• Operon: ypjD-dapB-mgsA-bshB1-bshA-cca-birA PubMed

• Expression browser: dapB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by diamide stess (thiol depletion) (Spx) PubMed

• Regulatory mechanism:
  • Spx: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Operon and expression

Ahmed Gaballa, Haike Antelmann, Chris J Hamilton, John D Helmann
Regulation of Bacillus subtilis bacillithiol biosynthesis operons by Spx.
Microbiology (Reading): 2013, 159(Pt 10);2025-2035
[PubMed:23894131] [WorldCat.org] [DOI] (I p)

Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541] [WorldCat.org] [DOI] (I p)