ManR

• Description: transcriptional activator of the manP-manA-yjdF operon
  
  

Gene name	manR
Synonyms	yjdC
Essential	no
Product	transcriptional activator, PRD-type
Function	regulation of mannose utilization
Gene expression levels in SubtiExpress: manR
Interactions involving this protein in SubtInteract: ManR
Metabolic function and regulation of this protein in SubtiPathways: manR
MW, pI	75 kDa, 8.04
Gene length, protein length	1944 bp, 648 aa
Immediate neighbours	yjdB, manP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, ManR regulon

The ManR regulon:

• manP-manA-yjdF, manR

The gene

Basic information

• Locus tag: BSU12000

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation by ManP inactivates ManR (in the absence of mannose), phosphorylation by HPr stimluates ManR PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ManP-ManR PubMed
  • HPr-ManR PubMed

• Localization:

Database entries

• Structure:

• UniProt: O31644

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: manR PubMed

• Expression browser: manR PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by mannose (ManR) PubMed
  • repressed by glucose (CcpA, ManR) PubMed

• Regulatory mechanism:
  • ManR: transcription activation in the presence of mannose and absence of glucose PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Marian Wenzel, Josef Altenbuchner
The Bacillus subtilis mannose regulator, ManR, a DNA-binding protein regulated by HPr and its cognate PTS transporter ManP.
Mol Microbiol: 2013, 88(3);562-76
[PubMed:23551403] [WorldCat.org] [DOI] (I p)

Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538] [WorldCat.org] [DOI] (I e)

Tianqi Sun, Josef Altenbuchner
Characterization of a mannose utilization system in Bacillus subtilis.
J Bacteriol: 2010, 192(8);2128-39
[PubMed:20139185] [WorldCat.org] [DOI] (I p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)

J Stülke, M Arnaud, G Rapoport, I Martin-Verstraete
PRD--a protein domain involved in PTS-dependent induction and carbon catabolite repression of catabolic operons in bacteria.
Mol Microbiol: 1998, 28(5);865-74
[PubMed:9663674] [WorldCat.org] [DOI] (P p)