Difference between revisions of "SafA"
| Line 80: | Line 80: | ||
* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
| + | ** contains four N-acetylglucosamine-polymer-binding [[LysM domain]]s {{PubMed|18430080}} | ||
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| Line 112: | Line 113: | ||
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=safA_2844675_2845838_-1 safA] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=safA_2844675_2845838_-1 safA] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigE]] {{PubMed|10438771}} | + | * '''[[Sigma factor]]:''' [[SigE]] {{PubMed|10438771}} |
* '''Regulation:''' | * '''Regulation:''' | ||
| Line 145: | Line 146: | ||
=References= | =References= | ||
| − | == Reviews == | + | == Reviews== |
| − | <pubmed> 22192522 </pubmed> | + | <pubmed>18430080 22192522 </pubmed> |
== Original publications == | == Original publications == | ||
<pubmed>10438771,16950916,11222602,11325931, 20451384,10714986, 22171814 22262582 23811766 </pubmed> | <pubmed>10438771,16950916,11222602,11325931, 20451384,10714986, 22171814 22262582 23811766 </pubmed> | ||
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 14:47, 27 December 2013
- Description: morphogenetic protein associated with SpoVID, major organizer of the inner spore coat
| Gene name | safA |
| Synonyms | yrbA |
| Essential | no |
| Product | morphogenetic protein |
| Function | spore coat formation |
| Gene expression levels in SubtiExpress: safA | |
| Interactions involving this protein in SubtInteract: SafA | |
| MW, pI | 43 kDa, 5.753 |
| Gene length, protein length | 1161 bp, 387 aa |
| Immediate neighbours | coxA, nadA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
SafA-dependent proteins of the inner spore coat
- CotD, CotP, CotT, CwlJ, OxdD, YaaH, YeeK, YisY, YhjR, YjqC, YmaG, YsnD, YsxE, YutH, YuzC, YxeE, YybI PubMed
The gene
Basic information
- Locus tag: BSU27840
Phenotypes of a mutant
- mis-assembly of the inner spore coat PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- contains four N-acetylglucosamine-polymer-binding LysM domains PubMed
- Modification:
- Effectors of protein activity:
- Localization: spore wall (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O32062
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
- Adriano Henriques, Lisbon, Portugal homepage
- Charles Moran, Emory University, NC, USA homepage
Your additional remarks
References
Reviews
Peter Setlow
Dynamics of the assembly of a complex macromolecular structure--the coat of spores of the bacterium Bacillus subtilis.
Mol Microbiol: 2012, 83(2);241-4
[PubMed:22192522]
[WorldCat.org]
[DOI]
(I p)
Girbe Buist, Anton Steen, Jan Kok, Oscar P Kuipers
LysM, a widely distributed protein motif for binding to (peptido)glycans.
Mol Microbiol: 2008, 68(4);838-47
[PubMed:18430080]
[WorldCat.org]
[DOI]
(I p)
Original publications
Kazuhito Watabe
[Overview of study on Bacillus subtilis spores].
Yakugaku Zasshi: 2013, 133(7);783-97
[PubMed:23811766]
[WorldCat.org]
[DOI]
(I p)
Haiyan Qiao, Daniela Krajcikova, Caisheng Liu, Yongjun Li, Hongda Wang, Imrich Barak, Jilin Tang
The interactions of spore-coat morphogenetic proteins studied by single-molecule recognition force spectroscopy.
Chem Asian J: 2012, 7(4);725-31
[PubMed:22262582]
[WorldCat.org]
[DOI]
(I p)
Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814]
[WorldCat.org]
[DOI]
(I p)
Peter T McKenney, Adam Driks, Haig A Eskandarian, Paul Grabowski, Jonathan Guberman, Katherine H Wang, Zemer Gitai, Patrick Eichenberger
A distance-weighted interaction map reveals a previously uncharacterized layer of the Bacillus subtilis spore coat.
Curr Biol: 2010, 20(10);934-8
[PubMed:20451384]
[WorldCat.org]
[DOI]
(I p)
Teresa Costa, Anabela L Isidro, Charles P Moran, Adriano O Henriques
Interaction between coat morphogenetic proteins SafA and SpoVID.
J Bacteriol: 2006, 188(22);7731-41
[PubMed:16950916]
[WorldCat.org]
[DOI]
(P p)
A J Ozin, C S Samford, A O Henriques, C P Moran
SpoVID guides SafA to the spore coat in Bacillus subtilis.
J Bacteriol: 2001, 183(10);3041-9
[PubMed:11325931]
[WorldCat.org]
[DOI]
(P p)
A J Ozin, T Costa, A O Henriques, C P Moran
Alternative translation initiation produces a short form of a spore coat protein in Bacillus subtilis.
J Bacteriol: 2001, 183(6);2032-40
[PubMed:11222602]
[WorldCat.org]
[DOI]
(P p)
A J Ozin, A O Henriques, H Yi, C P Moran
Morphogenetic proteins SpoVID and SafA form a complex during assembly of the Bacillus subtilis spore coat.
J Bacteriol: 2000, 182(7);1828-33
[PubMed:10714986]
[WorldCat.org]
[DOI]
(P p)
H Takamatsu, T Kodama, T Nakayama, K Watabe
Characterization of the yrbA gene of Bacillus subtilis, involved in resistance and germination of spores.
J Bacteriol: 1999, 181(16);4986-94
[PubMed:10438771]
[WorldCat.org]
[DOI]
(P p)
