RecU

• Description: DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates RecA activities, equivalent to E. coli RecU
  
  

Gene name	recU
Synonyms	recG, prfA, yppB, jopB
Essential	no
Product	Holliday junction resolvase
Function	DNA repair, homologous recombination and chromosome segregation
Gene expression levels in SubtiExpress: recU
Interactions involving this protein in SubtInteract: RecU
MW, pI	23 kDa, 9.511
Gene length, protein length	618 bp, 206 aa
Immediate neighbours	yppC, ponA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, cell envelope stress proteins (controlled by SigM, V, W, X, Y)

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

• Locus tag: BSU22310

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • form dimers PubMed
  • RecA-RecU PubMed
  • RecU-RuvB PubMed

• Localization:

Database entries

• Structure: 1ZP7

• UniProt: P39792

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: recU-ponA PubMed

• Expression browser: recU PubMed

• Sigma factor: SigM PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP891 (recU::cat), available in Jörg Stülke's lab
  • 1A895 (no resistance), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

S C West
Processing of recombination intermediates by the RuvABC proteins.
Annu Rev Genet: 1997, 31;213-44
[PubMed:9442895] [WorldCat.org] [DOI] (P p)

Original publications

Cristina Cañas, Begoña Carrasco, Esther García-Tirado, John B Rafferty, Juan C Alonso, Silvia Ayora
The stalk region of the RecU resolvase is essential for Holliday junction recognition and distortion.
J Mol Biol: 2011, 410(1);39-49
[PubMed:21600217] [WorldCat.org] [DOI] (I p)

Dawit Kidane, Begoña Carrasco, Candela Manfredi, Katharina Rothmaier, Silvia Ayora, Serkalem Tadesse, Juan C Alonso, Peter L Graumann
Evidence for different pathways during horizontal gene transfer in competent Bacillus subtilis cells.
PLoS Genet: 2009, 5(9);e1000630
[PubMed:19730681] [WorldCat.org] [DOI] (I p)

Begoña Carrasco, Cristina Cañas, Gary J Sharples, Juan C Alonso, Silvia Ayora
The N-terminal region of the RecU holliday junction resolvase is essential for homologous recombination.
J Mol Biol: 2009, 390(1);1-9
[PubMed:19422832] [WorldCat.org] [DOI] (I p)

Cristina Cañas, Begoña Carrasco, Silvia Ayora, Juan C Alonso
The RecU Holliday junction resolvase acts at early stages of homologous recombination.
Nucleic Acids Res: 2008, 36(16);5242-9
[PubMed:18684995] [WorldCat.org] [DOI] (I p)

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)

Natalie McGregor, Sylvia Ayora, Svetlana Sedelnikova, Begona Carrasco, Juan C Alonso, Paul Thaw, John Rafferty
The structure of Bacillus subtilis RecU Holliday junction resolvase and its role in substrate selection and sequence-specific cleavage.
Structure: 2005, 13(9);1341-51
[PubMed:16154091] [WorldCat.org] [DOI] (P p)

Begoña Carrasco, Silvia Ayora, Rudi Lurz, Juan C Alonso
Bacillus subtilis RecU Holliday-junction resolvase modulates RecA activities.
Nucleic Acids Res: 2005, 33(12);3942-52
[PubMed:16024744] [WorldCat.org] [DOI] (I e)

Humberto Sanchez, Dawit Kidane, Patricia Reed, Fiona A Curtis, M Castillo Cozar, Peter L Graumann, Gary J Sharples, Juan C Alonso
The RuvAB branch migration translocase and RecU Holliday junction resolvase are required for double-stranded DNA break repair in Bacillus subtilis.
Genetics: 2005, 171(3);873-83
[PubMed:16020779] [WorldCat.org] [DOI] (P p)

Begoña Carrasco, M Castillo Cozar, Rudi Lurz, Juan C Alonso, Silvia Ayora
Genetic recombination in Bacillus subtilis 168: contribution of Holliday junction processing functions in chromosome segregation.
J Bacteriol: 2004, 186(17);5557-66
[PubMed:15317759] [WorldCat.org] [DOI] (P p)

B Carrasco, S Fernández, K Asai, N Ogasawara, J C Alonso
Effect of the recU suppressors sms and subA on DNA repair and homologous recombination in Bacillus subtilis.
Mol Genet Genomics: 2002, 266(5);899-906
[PubMed:11810266] [WorldCat.org] [DOI] (P p)

S Fernández, A Sorokin, J C Alonso
Genetic recombination in Bacillus subtilis 168: effects of recU and recS mutations on DNA repair and homologous recombination.
J Bacteriol: 1998, 180(13);3405-9
[PubMed:9642195] [WorldCat.org] [DOI] (P p)

D L Popham, P Setlow
Cloning, nucleotide sequence, and mutagenesis of the Bacillus subtilis ponA operon, which codes for penicillin-binding protein (PBP) 1 and a PBP-related factor.
J Bacteriol: 1995, 177(2);326-35
[PubMed:7814321] [WorldCat.org] [DOI] (P p)

RecU in other organisms

Ana R Pereira, Patricia Reed, Helena Veiga, Mariana G Pinho
The Holliday junction resolvase RecU is required for chromosome segregation and DNA damage repair in Staphylococcus aureus.
BMC Microbiol: 2013, 13;18
[PubMed:23356868] [WorldCat.org] [DOI] (I e)