Difference between revisions of "DegU"
| Line 60: | Line 60: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
* defect in [[biofilm formation]] {{PubMed|20815827}}, this can be suppressed by DegU-independent expression of [[YuaB]] {{PubMed|21742882}} | * defect in [[biofilm formation]] {{PubMed|20815827}}, this can be suppressed by DegU-independent expression of [[YuaB]] {{PubMed|21742882}} | ||
| + | * the mutation suppresses the mucoid phenotype of ''[[motA]]'' or ''[[motB]]'' mutants due to reduced expression of the ''[[pgsB]]-[[pgsC]]-[[pgsA]]'' operon {{PubMed|24296669}} | ||
| + | |||
=== Database entries === | === Database entries === | ||
| Line 82: | Line 84: | ||
* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' phosphorylated by [[DegS]] on Asp-56 [http://www.ncbi.nlm.nih.gov/sites/entrez/1901568 PubMed], this modulates DNA-binding activity | * '''Modification:''' phosphorylated by [[DegS]] on Asp-56 [http://www.ncbi.nlm.nih.gov/sites/entrez/1901568 PubMed], this modulates DNA-binding activity | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
| Line 120: | Line 122: | ||
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=degU_3644607_3645296_-1 degU] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=degU_3644607_3645296_-1 degU] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
** ''[[degS]]'': [[SigA]] {{PubMed|1688843}} | ** ''[[degS]]'': [[SigA]] {{PubMed|1688843}} | ||
** ''[[degU]]'': [[SigA]] {{PubMed|18502860}} | ** ''[[degU]]'': [[SigA]] {{PubMed|18502860}} | ||
| Line 161: | Line 163: | ||
<pubmed>12471443,,</pubmed> | <pubmed>12471443,,</pubmed> | ||
==Original Publications== | ==Original Publications== | ||
| − | <pubmed>17850253,8878039, 14563871,1901568,1321152,1459944, 18194340 18978066 10908654,18502860,10094672,19389763, 20815827 21742882 18414485,19420703,2428811, 7746142, 18502860, 19389763, 19416356, 19734658 1688843 20070525 18197985 15598897 12950930 17590234 8955341 22496484 22745669 23123903 21965392 24123822 </pubmed> | + | <pubmed>17850253,8878039, 14563871,1901568,1321152,1459944, 18194340 18978066 10908654,18502860,10094672,19389763, 20815827 21742882 18414485,19420703,2428811, 7746142, 18502860, 19389763, 19416356, 19734658 1688843 20070525 18197985 15598897 12950930 17590234 8955341 22496484 22745669 23123903 21965392 24123822 24296669</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 20:09, 4 December 2013
- Description: two-component response regulator, regulation of degradative enzyme and genetic competence, involved in activation of capsule biosynthetic operon expression (together with SwrAA), non-phosphorylated DegU is required for swarming motility PubMed
| Gene name | degU |
| Synonyms | sacU, iep |
| Essential | no |
| Product | two-component response regulator |
| Function | regulation of degradative enzyme and genetic competence |
| Gene expression levels in SubtiExpress: degU | |
| Interactions involving this protein in SubtInteract: DegU | |
| Metabolic function and regulation of this protein in SubtiPathways: Protein secretion | |
| MW, pI | 25 kDa, 5.602 |
| Gene length, protein length | 687 bp, 229 aa |
| Immediate neighbours | yviA, degS |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biofilm formation, transcription factors and their control, phosphoproteins
This gene is a member of the following regulons
CcpA regulon, DegU regulon, TnrA regulon
The DegU regulon
The gene
Basic information
- Locus tag: BSU35490
Phenotypes of a mutant
- defect in biofilm formation PubMed, this can be suppressed by DegU-independent expression of YuaB PubMed
- the mutation suppresses the mucoid phenotype of motA or motB mutants due to reduced expression of the pgsB-pgsC-pgsA operon PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: transcription activator of degradative enzyme genes (sacB and degQ) and of comK expression
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P13800
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Robert Belas
When the swimming gets tough, the tough form a biofilm.
Mol Microbiol: 2013, 90(1);1-5
[PubMed:23927648]
[WorldCat.org]
[DOI]
(I p)
Daniel López, Roberto Kolter
Extracellular signals that define distinct and coexisting cell fates in Bacillus subtilis.
FEMS Microbiol Rev: 2010, 34(2);134-49
[PubMed:20030732]
[WorldCat.org]
[DOI]
(I p)
Ewan J Murray, Taryn B Kiley, Nicola R Stanley-Wall
A pivotal role for the response regulator DegU in controlling multicellular behaviour.
Microbiology (Reading): 2009, 155(Pt 1);1-8
[PubMed:19118340]
[WorldCat.org]
[DOI]
(P p)
The DegU regulon
U Mäder, H Antelmann, T Buder, M K Dahl, M Hecker, G Homuth
Bacillus subtilis functional genomics: genome-wide analysis of the DegS-DegU regulon by transcriptomics and proteomics.
Mol Genet Genomics: 2002, 268(4);455-67
[PubMed:12471443]
[WorldCat.org]
[DOI]
(P p)
Original Publications
