Difference between revisions of "KtrA"
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===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| − | The '' | + | * The ''[[ktrA]]-[[ktrB]]'' mutant of ''B. subtilis'' NCIB3610 is reduced in sliding (dendritic spreading) {{PubMed|16321950}} |
=== Database entries === | === Database entries === | ||
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=== Additional information=== | === Additional information=== | ||
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* '''Domains:''' | * '''Domains:''' | ||
| + | ** contains a [[RCK_N domain]] at the N-terminus (according to UniProt, [http://www.uniprot.org/uniprot/?query=domain:%22RCK+N-terminal+domain*%22]) | ||
** contains a c-di-AMP-binding [[RCK_C domain]] at the C-terminus (according to UniProt, [http://www.uniprot.org/uniprot/?query=domain:%22RCK+C-terminal+domain*%22]) {{PubMed|23671116}} | ** contains a c-di-AMP-binding [[RCK_C domain]] at the C-terminus (according to UniProt, [http://www.uniprot.org/uniprot/?query=domain:%22RCK+C-terminal+domain*%22]) {{PubMed|23671116}} | ||
| + | |||
* '''Modification:''' | * '''Modification:''' | ||
Revision as of 18:16, 4 December 2013
- Description: high affinity potassium transporter KtrA-KtrB, peripheric membrane component (proton symport)
| Gene name | ktrA |
| Synonyms | yuaA |
| Essential | no |
| Product | high affinity potassium transporter KtrA-KtrB, peripheric membrane component (proton symport) |
| Function | potassium uptake |
| Gene expression levels in SubtiExpress: ktrA | |
| Interactions involving this protein in SubtInteract: KtrA | |
| Metabolic function and regulation of this protein in SubtiPathways: Metal ion homeostasis, Stress | |
| MW, pI | 24 kDa, 5.981 |
| Gene length, protein length | 666 bp, 222 aa |
| Immediate neighbours | bslA, ktrB |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), coping with hyper-osmotic stress, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU31090
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s): KtrC
Extended information on the protein
- Kinetic information:
- Domains:
- contains a RCK_N domain at the N-terminus (according to UniProt, [2])
- contains a c-di-AMP-binding RCK_C domain at the C-terminus (according to UniProt, [3]) PubMed
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- the protein binds c-di-AMP PubMed
- Localization: peripheral membrane protein PubMed
Database entries
- UniProt: O32080
- KEGG entry: [4]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- induced if the energy charge is low (ydaO riboswitch) PubMed
- Regulatory mechanism:
- expression is controlled via termination antitermination by the ydaO riboswitch PubMed
- Additional information:
Biological materials
- Mutant:
- 1A954 ( ktrA::kan), PubMed, available at BGSC
- GHB1 (D(ktrA-ktrB)::aphA3), available in Erhard Bremer's lab
- GP92 (D(ktrA-ktrB)::aphA3), available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Erhard Bremer, University of Marburg, Germany homepage
Your additional remarks
References
