TsaD

• Description: required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA (together with TsaC), universally conserved protein
  
  

Gene name	tsaD
Synonyms	ydiE, gcp
Essential	yes PubMed
Product	tRNA modification enzyme
Function	tRNA modification
Gene expression levels in SubtiExpress: tsaD
Interactions involving this protein in SubtInteract: TsaD
MW, pI	36 kDa, 5.016
Gene length, protein length	1038 bp, 346 aa
Immediate neighbours	ydiD, ydiF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, universally conserved proteins

The gene

Basic information

• Locus tag: BSU05940

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP (TsaB-TsaD-TsaE) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • TsaB-TsaD PubMed
  • TsaD-TsaE PubMed

• Localization:

Database entries

• Structure:

• UniProt: O05518

• KEGG entry: [2]

• E.C. number:

Additional information

universally conserved protein

Expression and regulation

• Operon: thiL-tsaE-tsaB-ydiD-tsaD PubMed

• Expression browser: tsaD PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Charles T Lauhon
Mechanism of N6-threonylcarbamoyladenonsine (t(6)A) biosynthesis: isolation and characterization of the intermediate threonylcarbamoyl-AMP.
Biochemistry: 2012, 51(44);8950-63
[PubMed:23072323] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Publications on the corresponding protein from other species

Ting Lei, Junshu Yang, Li Zheng, Todd Markowski, Bruce A Witthuhn, Yinduo Ji
The essentiality of staphylococcal Gcp is independent of its repression of branched-chain amino acids biosynthesis.
PLoS One: 2012, 7(10);e46836
[PubMed:23056478] [WorldCat.org] [DOI] (I p)

Ting Lei, Xudong Liang, Junshu Yang, Meiying Yan, Li Zheng, Bruce Walcheck, Yinduo Ji
The C-terminal domain of the novel essential protein Gcp is critical for interaction with another essential protein YeaZ of Staphylococcus aureus.
PLoS One: 2011, 6(5);e20163
[PubMed:21625506] [WorldCat.org] [DOI] (I p)

Tobias Bergmiller, Rafael Peña-Miller, Alexander Boehm, Martin Ackermann
Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD.
BMC Microbiol: 2011, 11;118
[PubMed:21619589] [WorldCat.org] [DOI] (I e)

Basma El Yacoubi, Isabelle Hatin, Christopher Deutsch, Tamer Kahveci, Jean-Pierre Rousset, Dirk Iwata-Reuyl, Alexey G Murzin, Valérie de Crécy-Lagard
A role for the universal Kae1/Qri7/YgjD (COG0533) family in tRNA modification.
EMBO J: 2011, 30(5);882-93
[PubMed:21285948] [WorldCat.org] [DOI] (I p)

Madhusudhan Srinivasan, Preeti Mehta, Yao Yu, Evelyn Prugar, Eugene V Koonin, A Wali Karzai, Rolf Sternglanz
The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A.
EMBO J: 2011, 30(5);873-81
[PubMed:21183954] [WorldCat.org] [DOI] (I p)

Chen Katz, Ifat Cohen-Or, Uri Gophna, Eliora Z Ron
The ubiquitous conserved glycopeptidase Gcp prevents accumulation of toxic glycated proteins.
mBio: 2010, 1(3);
[PubMed:20824107] [WorldCat.org] [DOI] (I e)

Jennifer I Handford, Bérengère Ize, Grant Buchanan, Gareth P Butland, Jack Greenblatt, Andrew Emili, Tracy Palmer
Conserved network of proteins essential for bacterial viability.
J Bacteriol: 2009, 191(15);4732-49
[PubMed:19376873] [WorldCat.org] [DOI] (I p)