NatR

• Description: two-component response regulator, regulation of the natA-natB operon
  
  

Gene name	natR
Synonyms	yccH
Essential	no
Product	two-component response regulator
Function	regulation of the natA-natB operon
Gene expression levels in SubtiExpress: natR
Interactions involving this protein in SubtInteract: NatR
MW, pI	26 kDa, 6.614
Gene length, protein length	699 bp, 233 aa
Immediate neighbours	natK, natA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

metal ion homeostasis (K, Na, Ca, Mg), transcription factors and their control, phosphoproteins

The NatR regulon:

• natA-natB

The gene

Basic information

• Locus tag: BSU02740

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: OmpR family of two-component response regulators

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated by NatK on an Asp residue

• Cofactor(s):

• Effectors of protein activity: phosphorylation likely affects DNA-binding activity

• Interactions:
  • NatK-NatR

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P70955

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: natR (according to DBTBS)

• Expression browser: natR PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Mitsuo Ogura, Kensuke Tsukahara, Kentaro Hayashi, Teruo Tanaka
The Bacillus subtilis NatK-NatR two-component system regulates expression of the natAB operon encoding an ABC transporter for sodium ion extrusion.
Microbiology (Reading): 2007, 153(Pt 3);667-675
[PubMed:17322186] [WorldCat.org] [DOI] (P p)

C Fabret, V A Feher, J A Hoch
Two-component signal transduction in Bacillus subtilis: how one organism sees its world.
J Bacteriol: 1999, 181(7);1975-83
[PubMed:10094672] [WorldCat.org] [DOI] (P p)