RtpA

• Description: anti-TRAP
  
  

Gene name	rtpA
Synonyms	yczA
Essential	no
Product	anti-TRAP
Function	regulation of tryptophan biosynthesis
Gene expression levels in SubtiExpress: rtpA
Interactions involving this protein in SubtInteract: RtpA
Metabolic function and regulation of this protein in SubtiPathways: Phe, Tyr, Trp
MW, pI	5 kDa, 4.862
Gene length, protein length	159 bp, 53 aa
Immediate neighbours	ycbJ, ycbK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, transcription factors and their control

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU02530

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • TRAP-RtpA PubMed
  • forms trimers and dodecamers (tetramer of trimers) PubMed, only the trimeric form interacts with TRAP PubMed

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1N53 (structure of T box RNA), 2BX9, 2ZP9 (complex with MtrB)

• UniProt: O31466

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rtpA-ycbK PubMed

• Expression browser: rtpA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by tryptophan limitation (T-box in the mRNA leader, TRAP) PubMed

• Regulatory mechanism:
  • T-box, TRAP: transcription termination/ antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Paul Gollnick, Paul Babitzke, Alfred Antson, Charles Yanofsky
Complexity in regulation of tryptophan biosynthesis in Bacillus subtilis.
Annu Rev Genet: 2005, 39;47-68
[PubMed:16285852] [WorldCat.org] [DOI] (P p)