DnaD

• Description: initiation of chromosome replication, part of the replisome
  
  

Gene name	dnaD
Synonyms
Essential	yes PubMed
Product	initiation of chromosome replication
Function	DNA replication
Gene expression levels in SubtiExpress: dnaD
Interactions involving this protein in SubtInteract: DnaD
MW, pI	27 kDa, 7.174
Gene length, protein length	696 bp, 232 aa
Immediate neighbours	nth, asnS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, essential genes

The gene

Basic information

• Locus tag: BSU22350

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • DnaD-mediated untwisting of the DNA stimulates Nth endonuclease activity at AP sites PubMed
  • required for the assembly of the replicative helicase DnaC at the origin of replication PubMed
  • interaction with DnaA inhibits the ability of DnaA to bind cooperatively to DNA PubMed

• Protein family: DNA polymerase type-Y family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DnaD-PriA PubMed
  • DnaD-DnaA PubMed
  • DnaD-DnaB PubMed
  • part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
  • part of the primosome: DnaA-DnaG-DnaC-DnaI-DnaD-DnaB PubMed

• Localization: Membrane-proximal (Spotty) PubMed

Database entries

• Structure: 2C79 (N-terminal domain)

• UniProt: P39787

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: dnaD-nth PubMed

• Expression browser: dnaD PubMed

• Sigma factor:

• Regulation:
  • constitutively expressed PubMed
  • there are about 3,000 to 5,000 molecules of DnaD per cell PubMed
  • expression is transiently increased after treatment with H2O2 PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Alan Grossman, MIT, Cambridge, MA, USA

References

Reviews

Geoffrey S Briggs, Wiep Klaas Smits, Panos Soultanas
Chromosomal replication initiation machinery of low-G+C-content Firmicutes.
J Bacteriol: 2012, 194(19);5162-70
[PubMed:22797751] [WorldCat.org] [DOI] (I p)