SigB

• Description: RNA polymerase sigma factor SigB
  
  

Gene name	sigB
Synonyms	rpoF
Essential	no
Product	RNA polymerase sigma factor SigB
Function	general stress response
Gene expression levels in SubtiExpress: sigB
Interactions involving this protein in SubtInteract: SigB
Metabolic function and regulation of this protein in SubtiPathways: Stress, Murein recycling
MW, pI	29 kDa, 5.418
Gene length, protein length	792 bp, 264 aa
Immediate neighbours	rsbW, rsbX
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transcription, sigma factors and their control, general stress proteins (controlled by SigB)

This gene is a member of the following regulons

CcpA regulon, SigB regulon

The gene

Basic information

• Locus tag: BSU04730

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: SigB subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SigB-RsbW PubMed
  • SigB-RpoB-RpoC

• Localization:

Database entries

• Structure:

• UniProt: P06574

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • rsbR-rsbS-rsbT-rsbU-rsbV-rsbW-sigB-rsbX PubMed
  • rsbV-rsbW-sigB-rsbX PubMed

• Expression browser: sigB PubMed

• Sigma factor:
  • rsbR: SigA PubMed
  • rsbV: SigB PubMed

• Regulation:
  • rsbV: induced by stress (SigB) PubMed

• Additional information:

Biological materials

• Mutant: QB5344 (cat), available in the Stülke lab
  • 1A675 ( sigB::cat), PubMed, available at BGSC
  • 1A777 ( sigB::cat), PubMed, available at BGSC
  • 1A780 ( sigB::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Bill Haldenwang, San Antonio, USA
• Chet Price, Davis, USA homepage

References

Identification of the SigB regulon

Priyanka Nannapaneni, Falk Hertwig, Maren Depke, Michael Hecker, Ulrike Mäder, Uwe Völker, Leif Steil, Sacha A F T van Hijum
Defining the structure of the general stress regulon of Bacillus subtilis using targeted microarray analysis and random forest classification.
Microbiology (Reading): 2012, 158(Pt 3);696-707
[PubMed:22174379] [WorldCat.org] [DOI] (I p)

J D Helmann, M F Wu, P A Kobel, F J Gamo, M Wilson, M M Morshedi, M Navre, C Paddon
Global transcriptional response of Bacillus subtilis to heat shock.
J Bacteriol: 2001, 183(24);7318-28
[PubMed:11717291] [WorldCat.org] [DOI] (P p)

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)

C W Price, P Fawcett, H Cérémonie, N Su, C K Murphy, P Youngman
Genome-wide analysis of the general stress response in Bacillus subtilis.
Mol Microbiol: 2001, 41(4);757-74
[PubMed:11532142] [WorldCat.org] [DOI] (P p)

A Petersohn, J Bernhardt, U Gerth, D Höper, T Koburger, U Völker, M Hecker
Identification of sigma(B)-dependent genes in Bacillus subtilis using a promoter consensus-directed search and oligonucleotide hybridization.
J Bacteriol: 1999, 181(18);5718-24
[PubMed:10482513] [WorldCat.org] [DOI] (P p)

Anja Petersohn, Haike Antelmann, Ulf Gerth, Michael Hecker
Identification and transcriptional analysis of new members of the sigmaB regulon in Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 4);869-880
[PubMed:10220166] [WorldCat.org] [DOI] (P p)

Other publications

Chun-Kai Yang, Phang C Tai, Chung-Dar Lu
Time-related transcriptome analysis of B. subtilis 168 during growth with glucose.
Curr Microbiol: 2014, 68(1);12-20
[PubMed:23934352] [WorldCat.org] [DOI] (I p)

Daina L Ringus, Ahmed Gaballa, John D Helmann, Martin Wiedmann, Kathryn J Boor
Fluoro-phenyl-styrene-sulfonamide, a novel inhibitor of σB activity, prevents the activation of σB by environmental and energy stresses in Bacillus subtilis.
J Bacteriol: 2013, 195(11);2509-17
[PubMed:23524614] [WorldCat.org] [DOI] (I p)

Jonathan W Young, James C W Locke, Michael B Elowitz
Rate of environmental change determines stress response specificity.
Proc Natl Acad Sci U S A: 2013, 110(10);4140-5
[PubMed:23407164] [WorldCat.org] [DOI] (I p)

Alexander Reder, Dirk Höper, Ulf Gerth, Michael Hecker
Contributions of individual σB-dependent general stress genes to oxidative stress resistance of Bacillus subtilis.
J Bacteriol: 2012, 194(14);3601-10
[PubMed:22582280] [WorldCat.org] [DOI] (I p)

Ulf W Liebal, Praveen K Sappa, Thomas Millat, Leif Steil, Georg Homuth, Uwe Völker, Olaf Wolkenhauer
Proteolysis of beta-galactosidase following SigmaB activation in Bacillus subtilis.
Mol Biosyst: 2012, 8(6);1806-14
[PubMed:22511268] [WorldCat.org] [DOI] (I p)

Alexander Reder, Ulf Gerth, Michael Hecker
Integration of σB activity into the decision-making process of sporulation initiation in Bacillus subtilis.
J Bacteriol: 2012, 194(5);1065-74
[PubMed:22210769] [WorldCat.org] [DOI] (I p)

James C W Locke, Jonathan W Young, Michelle Fontes, María Jesús Hernández Jiménez, Michael B Elowitz
Stochastic pulse regulation in bacterial stress response.
Science: 2011, 334(6054);366-9
[PubMed:21979936] [WorldCat.org] [DOI] (I p)

Marcela Avila-Pérez, Jeroen B van der Steen, Remco Kort, Klaas J Hellingwerf
Red light activates the sigmaB-mediated general stress response of Bacillus subtilis via the energy branch of the upstream signaling cascade.
J Bacteriol: 2010, 192(3);755-62
[PubMed:19948797] [WorldCat.org] [DOI] (I p)

Adam Reeves, Ulf Gerth, Uwe Völker, W G Haldenwang
ClpP modulates the activity of the Bacillus subtilis stress response transcription factor, sigmaB.
J Bacteriol: 2007, 189(17);6168-75
[PubMed:17586624] [WorldCat.org] [DOI] (P p)

Noriko Suzuki, Naoki Takaya, Takayuki Hoshino, Akira Nakamura
Enhancement of a sigma(B)-dependent stress response in Bacillus subtilis by light via YtvA photoreceptor.
J Gen Appl Microbiol: 2007, 53(2);81-8
[PubMed:17575448] [WorldCat.org] [DOI] (P p)

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Gudrun Holtmann, Matthias Brigulla, Leif Steil, Alexandra Schütz, Karsta Barnekow, Uwe Völker, Erhard Bremer
RsbV-independent induction of the SigB-dependent general stress regulon of Bacillus subtilis during growth at high temperature.
J Bacteriol: 2004, 186(18);6150-8
[PubMed:15342585] [WorldCat.org] [DOI] (P p)

Karen Carniol, Tae-Jong Kim, Chester W Price, Richard Losick
Insulation of the sigmaF regulatory system in Bacillus subtilis.
J Bacteriol: 2004, 186(13);4390-4
[PubMed:15205443] [WorldCat.org] [DOI] (P p)

Thorsten Mascher, Neil G Margulis, Tao Wang, Rick W Ye, John D Helmann
Cell wall stress responses in Bacillus subtilis: the regulatory network of the bacitracin stimulon.
Mol Microbiol: 2003, 50(5);1591-604
[PubMed:14651641] [WorldCat.org] [DOI] (P p)

Shuyu Zhang, W G Haldenwang
RelA is a component of the nutritional stress activation pathway of the Bacillus subtilis transcription factor sigma B.
J Bacteriol: 2003, 185(19);5714-21
[PubMed:13129942] [WorldCat.org] [DOI] (P p)

Matthias Brigulla, Tamara Hoffmann, Andrea Krisp, Andrea Völker, Erhard Bremer, Uwe Völker
Chill induction of the SigB-dependent general stress response in Bacillus subtilis and its contribution to low-temperature adaptation.
J Bacteriol: 2003, 185(15);4305-14
[PubMed:12867438] [WorldCat.org] [DOI] (P p)

Claudia Rollenhagen, Haike Antelmann, Janine Kirstein, Olivier Delumeau, Michael Hecker, Michael D Yudkin
Binding of sigma(A) and sigma(B) to core RNA polymerase after environmental stress in Bacillus subtilis.
J Bacteriol: 2003, 185(1);35-40
[PubMed:12486038] [WorldCat.org] [DOI] (P p)

A Petersohn, S Engelmann, P Setlow, M Hecker
The katX gene of Bacillus subtilis is under dual control of sigmaB and sigmaF.
Mol Gen Genet: 1999, 262(1);173-9
[PubMed:10503549] [WorldCat.org] [DOI] (P p)

U Völker, B Maul, M Hecker
Expression of the sigmaB-dependent general stress regulon confers multiple stress resistance in Bacillus subtilis.
J Bacteriol: 1999, 181(13);3942-8
[PubMed:10383961] [WorldCat.org] [DOI] (P p)

T Schweder, A Kolyschkow, U Völker, M Hecker
Analysis of the expression and function of the sigmaB-dependent general stress regulon of Bacillus subtilis during slow growth.
Arch Microbiol: 1999, 171(6);439-43
[PubMed:10369900] [WorldCat.org] [DOI] (P p)

C von Blohn, B Kempf, R M Kappes, E Bremer
Osmostress response in Bacillus subtilis: characterization of a proline uptake system (OpuE) regulated by high osmolarity and the alternative transcription factor sigma B.
Mol Microbiol: 1997, 25(1);175-87
[PubMed:11902719] [WorldCat.org] [DOI] (P p)

S Alper, A Dufour, D A Garsin, L Duncan, R Losick
Role of adenosine nucleotides in the regulation of a stress-response transcription factor in Bacillus subtilis.
J Mol Biol: 1996, 260(2);165-77
[PubMed:8764398] [WorldCat.org] [DOI] (P p)

A R Redfield, C W Price
General stress transcription factor sigmaB of Bacillus subtilis is a stable protein.
J Bacteriol: 1996, 178(12);3668-70
[PubMed:8655572] [WorldCat.org] [DOI] (P p)

S A Boylan, A R Redfield, M S Brody, C W Price
Stress-induced activation of the sigma B transcription factor of Bacillus subtilis.
J Bacteriol: 1993, 175(24);7931-7
[PubMed:8253681] [WorldCat.org] [DOI] (P p)

A K Benson, W G Haldenwang
Regulation of sigma B levels and activity in Bacillus subtilis.
J Bacteriol: 1993, 175(8);2347-56
[PubMed:8468294] [WorldCat.org] [DOI] (P p)

A K Benson, W G Haldenwang
The sigma B-dependent promoter of the Bacillus subtilis sigB operon is induced by heat shock.
J Bacteriol: 1993, 175(7);1929-35
[PubMed:8458834] [WorldCat.org] [DOI] (P p)

C Ray, M Igo, W Shafer, R Losick, C P Moran
Suppression of ctc promoter mutations in Bacillus subtilis.
J Bacteriol: 1988, 170(2);900-7
[PubMed:3123466] [WorldCat.org] [DOI] (P p)

M Igo, M Lampe, C Ray, W Schafer, C P Moran, R Losick
Genetic studies of a secondary RNA polymerase sigma factor in Bacillus subtilis.
J Bacteriol: 1987, 169(8);3464-9
[PubMed:3112122] [WorldCat.org] [DOI] (P p)

M L Duncan, S S Kalman, S M Thomas, C W Price
Gene encoding the 37,000-dalton minor sigma factor of Bacillus subtilis RNA polymerase: isolation, nucleotide sequence, chromosomal locus, and cryptic function.
J Bacteriol: 1987, 169(2);771-8
[PubMed:3027048] [WorldCat.org] [DOI] (P p)

M M Igo, R Losick
Regulation of a promoter that is utilized by minor forms of RNA polymerase holoenzyme in Bacillus subtilis.
J Mol Biol: 1986, 191(4);615-24
[PubMed:3100810] [WorldCat.org] [DOI] (P p)

C Binnie, M Lampe, R Losick
Gene encoding the sigma 37 species of RNA polymerase sigma factor from Bacillus subtilis.
Proc Natl Acad Sci U S A: 1986, 83(16);5943-7
[PubMed:3016731] [WorldCat.org] [DOI] (P p)

W C Johnson, C P Moran, R Losick
Two RNA polymerase sigma factors from Bacillus subtilis discriminate between overlapping promoters for a developmentally regulated gene.
Nature: 1983, 302(5911);800-4
[PubMed:6405278] [WorldCat.org] [DOI] (P p)

J F Ollington, W G Haldenwang, T V Huynh, R Losick
Developmentally regulated transcription in a cloned segment of the Bacillus subtilis chromosome.
J Bacteriol: 1981, 147(2);432-42
[PubMed:6790515] [WorldCat.org] [DOI] (P p)

W G Haldenwang, R Losick
Novel RNA polymerase sigma factor from Bacillus subtilis.
Proc Natl Acad Sci U S A: 1980, 77(12);7000-4
[PubMed:6784117] [WorldCat.org] [DOI] (P p)

W G Haldenwang, R Losick
A modified RNA polymerase transcribes a cloned gene under sporulation control in Bacillus subtilis.
Nature: 1979, 282(5736);256-60
[PubMed:116131] [WorldCat.org] [DOI] (P p)