MreBH

• Description: cell shape-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex
  
  

Gene name	mreBH
Synonyms
Essential	no
Product	cell shape-determining protein
Function	cell shape determation
Gene expression levels in SubtiExpress: mreBH
Interactions involving this protein in SubtInteract: MreBH
MW, pI	35 kDa, 5.239
Gene length, protein length	1005 bp, 335 aa
Immediate neighbours	ykpC, abh
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell shape, heat shock proteins, membrane proteins

This gene is a member of the following regulons

SigI regulon

The gene

Basic information

• Locus tag: BSU14470

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • forms ring-shaped filaments in a heterologous system PubMed
  • required for the activity of LytE PubMed

• Protein family: ftsA/mreB family (according to Swiss-Prot)

• Paralogous protein(s): MreB, Mbl

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • part of the cell wall biosynthetic complex PubMed
  • MreBH-LytE PubMed
  • MreBH-Mbl PubMed
  • MreB-MreBH PubMed
  • RodZ-MreBH PubMed

• Localization:
  • during logarithmic growth, MreBH forms discrete patches thst move processively along peripheral tracks perpendicular to the cell axis PubMed
  • forms transverse bands as cells enter the stationary phase PubMed
  • close to the inner surface of the cytoplasmic membrane PubMed
  • reports on helical structures formed by MreBH PubMed seem to be misinterpretation of data PubMed
  • normal localization depends on the presence of glucolipids, MreB forms irregular clusters in an ugtP mutant PubMed

Database entries

• Structure:

• UniProt: P39763

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: mreBH PubMed

• Sigma factor: SigI PubMed

• Regulation:
  • induced by heat (SigI) PubMed
  • expression is increased in an ugtP mutant PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage

References

Localization

Satoshi Matsuoka, Minako Chiba, Yu Tanimura, Michihiro Hashimoto, Hiroshi Hara, Kouji Matsumoto
Abnormal morphology of Bacillus subtilis ugtP mutant cells lacking glucolipids.
Genes Genet Syst: 2011, 86(5);295-304
[PubMed:22362028] [WorldCat.org] [DOI] (I p)

Ethan C Garner, Remi Bernard, Wenqin Wang, Xiaowei Zhuang, David Z Rudner, Tim Mitchison
Coupled, circumferential motions of the cell wall synthesis machinery and MreB filaments in B. subtilis.
Science: 2011, 333(6039);222-5
[PubMed:21636745] [WorldCat.org] [DOI] (I p)

Julia Domínguez-Escobar, Arnaud Chastanet, Alvaro H Crevenna, Vincent Fromion, Roland Wedlich-Söldner, Rut Carballido-López
Processive movement of MreB-associated cell wall biosynthetic complexes in bacteria.
Science: 2011, 333(6039);225-8
[PubMed:21636744] [WorldCat.org] [DOI] (I p)

Henrik Strahl, Leendert W Hamoen
Membrane potential is important for bacterial cell division.
Proc Natl Acad Sci U S A: 2010, 107(27);12281-6
[PubMed:20566861] [WorldCat.org] [DOI] (I p)

Hervé Joël Defeu Soufo, Peter L Graumann
Dynamic localization and interaction with other Bacillus subtilis actin-like proteins are important for the function of MreB.
Mol Microbiol: 2006, 62(5);1340-56
[PubMed:17064365] [WorldCat.org] [DOI] (P p)