LexA

• Description: transcriptional repressor of the SOS regulon
  
  

Gene name	lexA
Synonyms	dinR
Essential	no
Product	transcriptional repressor of the SOS regulon
Function	regulation of DNA damage repair
Gene expression levels in SubtiExpress: lexA
Function and regulation of this protein in SubtiPathways: Phosphorelay, Protein secretion
MW, pI	22 kDa, 5.155
Gene length, protein length	615 bp, 205 aa
Immediate neighbours	fosB, yneA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, transcription factors and their control

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

• Locus tag: BSU17850

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Represses uvrB, dinB, dinC, recA genes and itself by binding to the 14 bp palindromic sequence 5'-CGAACNNNNGTTCG-3'. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair. (according to Swiss-Prot)

• Protein family: peptidase S24 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1JHH (from E. coli, S119A mutant, 33% identity, 52% similarity) PubMed

• UniProt: P31080

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: lexA PubMed

• Expression browser: lexA PubMed

• Sigma factor:

• Regulation:
  • induced upon DNA damage (LexA) PubMed

• Regulatory mechanism: autorepression
  • LexA: transcription repression PubMed

• Additional information:
  • A ncRNA is predicted between fosB and lexA PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)