MrpA

• Description: Na+ transporter subunit of the Na+/H+ antiporter, multiple resistance and pH homeostasis
  
  

Gene name	mrpA
Synonyms	yufT, shaA, ntrA
Essential	yes PubMed
Product	Na+/H+ antiporter subunit
Function	sodium export
Gene expression levels in SubtiExpress: mrpA
Interactions involving this protein in SubtInteract: MrpA
MW, pI	86 kDa, 9.519
Gene length, protein length	2322 bp, 774 aa
Immediate neighbours	yufS, mrpB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, metal ion homeostasis (K, Na, Ca, Mg), essential genes, membrane proteins

The gene

Basic information

• Locus tag: BSU31600

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Na+ transporter subunit of the Na+/H+ antiporter PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • MrpA-MrpB-MrpC-MrpD-MrpE-MrpF-MrpG (complex)

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: Q9K2S2

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mrpA-mrpB-mrpC-mrpD-mrpE-mrpF-mrpG PubMed

• Expression browser: mrpA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Egle Virzintiene, Vamsi K Moparthi, Yusra Al-Eryani, Leonard Shumbe, Kamil Górecki, Cecilia Hägerhäll
Structure and function of the C-terminal domain of MrpA in the Bacillus subtilis Mrp-antiporter complex--the evolutionary progenitor of the long horizontal helix in complex I.
FEBS Lett: 2013, 587(20);3341-7
[PubMed:24021651] [WorldCat.org] [DOI] (I p)

Vamsi K Moparthi, Brijesh Kumar, Cecilie Mathiesen, Cecilia Hägerhäll
Homologous protein subunits from Escherichia coli NADH:quinone oxidoreductase can functionally replace MrpA and MrpD in Bacillus subtilis.
Biochim Biophys Acta: 2011, 1807(4);427-36
[PubMed:21236240] [WorldCat.org] [DOI] (P p)

Yusuke Kajiyama, Masato Otagiri, Junichi Sekiguchi, Toshiaki Kudo, Saori Kosono
The MrpA, MrpB and MrpD subunits of the Mrp antiporter complex in Bacillus subtilis contain membrane-embedded and essential acidic residues.
Microbiology (Reading): 2009, 155(Pt 7);2137-2147
[PubMed:19389778] [WorldCat.org] [DOI] (P p)

Yusuke Kajiyama, Masato Otagiri, Junichi Sekiguchi, Saori Kosono, Toshiaki Kudo
Complex formation by the mrpABCDEFG gene products, which constitute a principal Na+/H+ antiporter in Bacillus subtilis.
J Bacteriol: 2007, 189(20);7511-4
[PubMed:17693497] [WorldCat.org] [DOI] (P p)

Saori Kosono, Yusuke Kajiyama, Shin Kawasaki, Toko Yoshinaka, Koki Haga, Toshiaki Kudo
Functional involvement of membrane-embedded and conserved acidic residues in the ShaA subunit of the multigene-encoded Na+/H+ antiporter in Bacillus subtilis.
Biochim Biophys Acta: 2006, 1758(5);627-35
[PubMed:16730649] [WorldCat.org] [DOI] (P p)

Saori Kosono, Kei Asai, Yoshito Sadaie, Toshiaki Kudo
Altered gene expression in the transition phase by disruption of a Na+/H+ antiporter gene (shaA) in Bacillus subtilis.
FEMS Microbiol Lett: 2004, 232(1);93-9
[PubMed:15019740] [WorldCat.org] [DOI] (P p)

M Ito, A A Guffanti, W Wang, T A Krulwich
Effects of nonpolar mutations in each of the seven Bacillus subtilis mrp genes suggest complex interactions among the gene products in support of Na(+) and alkali but not cholate resistance.
J Bacteriol: 2000, 182(20);5663-70
[PubMed:11004162] [WorldCat.org] [DOI] (P p)

M Ito, A A Guffanti, B Oudega, T A Krulwich
mrp, a multigene, multifunctional locus in Bacillus subtilis with roles in resistance to cholate and to Na+ and in pH homeostasis.
J Bacteriol: 1999, 181(8);2394-402
[PubMed:10198001] [WorldCat.org] [DOI] (P p)