Difference between revisions of "YdaP"
| Line 89: | Line 89: | ||
* '''Structure:''' | * '''Structure:''' | ||
| + | ** [http://www.pdb.org/pdb/explore/remediatedSequence.do?structureId=4KGD 4KGD] (the enzyme from Lactobacillus plantarum, 37% identity, 70% similarity) {{PubMed|23748673}} | ||
* '''UniProt:''' [http://www.uniprot.org/uniprot/P96591 P96591] | * '''UniProt:''' [http://www.uniprot.org/uniprot/P96591 P96591] | ||
| Line 104: | Line 105: | ||
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ydaP_488830_490554_1 ydaP] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ydaP_488830_490554_1 ydaP] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed] | + | * '''[[Sigma factor]]:''' [[SigB]] [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed] |
* '''Regulation:''' induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed] | * '''Regulation:''' induced by stress ([[SigB]]) [http://www.ncbi.nlm.nih.gov/pubmed/15805528 PubMed] | ||
| Line 132: | Line 133: | ||
=References= | =References= | ||
| − | <pubmed>10220166,,15805528, </pubmed> | + | <pubmed>10220166, 23748673,15805528, 22730460 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 10:31, 20 August 2013
- Description: general stress protein, required for survival of ethanol stress, putative pyruvate oxidase
| Gene name | ydaP |
| Synonyms | |
| Essential | no |
| Product | putative pyruvate oxidase |
| Function | unknown |
| Gene expression levels in SubtiExpress: ydaP | |
| MW, pI | 62 kDa, 5.217 |
| Gene length, protein length | 1722 bp, 574 aa |
| Immediate neighbours | mutT, ydzK |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
general stress proteins (controlled by SigB), poorly characterized/ putative enzymes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU04340
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: P96591
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: ydaP PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: GP457 (spc), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Danilo Meyer, Piotr Neumann, Ralf Ficner, Kai Tittmann
Observation of a stable carbene at the active site of a thiamin enzyme.
Nat Chem Biol: 2013, 9(8);488-90
[PubMed:23748673]
[WorldCat.org]
[DOI]
(I p)
Danilo Meyer, Piotr Neumann, Eline Koers, Hanno Sjuts, Stefan Lüdtke, George M Sheldrick, Ralf Ficner, Kai Tittmann
Unexpected tautomeric equilibria of the carbanion-enamine intermediate in pyruvate oxidase highlight unrecognized chemical versatility of thiamin.
Proc Natl Acad Sci U S A: 2012, 109(27);10867-72
[PubMed:22730460]
[WorldCat.org]
[DOI]
(I p)
Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528]
[WorldCat.org]
[DOI]
(P p)
Anja Petersohn, Haike Antelmann, Ulf Gerth, Michael Hecker
Identification and transcriptional analysis of new members of the sigmaB regulon in Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 4);869-880
[PubMed:10220166]
[WorldCat.org]
[DOI]
(P p)
