Difference between revisions of "DefB"
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|style="background:#ABCDEF;" align="center"|'''Gene name''' | |style="background:#ABCDEF;" align="center"|'''Gene name''' | ||
| − | |'' | + | |''defB'' |
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| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' | + | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ykrB'' |
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
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|style="background:#ABCDEF;" align="center"|'''Function''' || utilization of S-methyl-cysteine | |style="background:#ABCDEF;" align="center"|'''Function''' || utilization of S-methyl-cysteine | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14560 | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14560 defB] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 20 kDa, 5.456 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 20 kDa, 5.456 | ||
Revision as of 10:48, 18 August 2013
- Description: N-formylcysteine deformylase, required for the conversion of S-methyl-cysteine to cysteine
| Gene name | defB |
| Synonyms | ykrB |
| Essential | no |
| Product | N-formylcysteine deformylase |
| Function | utilization of S-methyl-cysteine |
| Gene expression levels in SubtiExpress: defB | |
| MW, pI | 20 kDa, 5.456 |
| Gene length, protein length | 552 bp, 184 aa |
| Immediate neighbours | ykrA, ykzV |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU14560
Phenotypes of a mutant
- no growth with S-methyl cysteine PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: N-acetylcystein --> cysteine + acetate PubMed
- Protein family: polypeptide deformylase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 1LQY (complex with antibiotic actinonin, Geobacillus stearothermophilus)
- UniProt: Q45495
- KEGG entry: [2]
- E.C. number: 3.5.1.88
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Che-Man Chan, Antoine Danchin, Philippe Marlière, Agnieszka Sekowska
Paralogous metabolism: S-alkyl-cysteine degradation in Bacillus subtilis.
Environ Microbiol: 2014, 16(1);101-17
[PubMed:23944997]
[WorldCat.org]
[DOI]
(I p)
Michael Haas, Dieter Beyer, Reinhold Gahlmann, Christoph Freiberg
YkrB is the main peptide deformylase in Bacillus subtilis, a eubacterium containing two functional peptide deformylases.
Microbiology (Reading): 2001, 147(Pt 7);1783-1791
[PubMed:11429456]
[WorldCat.org]
[DOI]
(P p)
