Difference between revisions of "CwlJ"
| Line 1: | Line 1: | ||
| − | * '''Description:''' cell wall hydrolase <br/><br/> | + | * '''Description:''' spore coat protein, cell wall hydrolase <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 10: | Line 10: | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || cell wall hydrolase | + | |style="background:#ABCDEF;" align="center"| '''Product''' || spore coat protein, cell wall hydrolase |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || spore germination | |style="background:#ABCDEF;" align="center"|'''Function''' || spore germination | ||
| Line 45: | Line 45: | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| − | {{SubtiWiki regulon|[[SigE regulon]]}} | + | {{SubtiWiki regulon|[[SigE regulon]]}}, |
| + | {{SubtiWiki regulon|[[SigK regulon]]}}, | ||
| + | {{SubtiWiki regulon|[[SpoIIID regulon]]}} | ||
=The gene= | =The gene= | ||
| Line 92: | Line 94: | ||
* '''[[Localization]]:''' | * '''[[Localization]]:''' | ||
** outer edge of the spore cortex {{PubMed|12177332}} | ** outer edge of the spore cortex {{PubMed|12177332}} | ||
| + | ** inner spore coat, localization depends on [[SafA]] {{PubMed|22171814}} | ||
=== Database entries === | === Database entries === | ||
| Line 111: | Line 114: | ||
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cwlJ_282469_282897_1 cwlJ] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cwlJ_282469_282897_1 cwlJ] {{PubMed|22383849}} | ||
| − | * '''[[Sigma factor]]:''' [[SigE]] {{PubMed|9515903,15699190}} | + | * '''[[Sigma factor]]:''' [[SigE]] {{PubMed|9515903,15699190}}, [[SigK]] {{PubMed|15383836}} |
* '''Regulation:''' | * '''Regulation:''' | ||
| − | ** expressed | + | ** expressed during sporulation in the mother cell ([[SigE]], [[SigK]], [[SpoIIID]]) {{PubMed|15383836,9515903,15699190}} |
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[SpoIIID]]: transcription activation {{PubMed|15383836}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 141: | Line 145: | ||
=References= | =References= | ||
| − | <pubmed>16936016,11807087,9515903,15699190,15317760,11466292,20435722 , 12177332 23543708 23746146</pubmed> | + | <pubmed>16936016,11807087,9515903,15699190,15317760,11466292,20435722 , 12177332 23543708 23746146 15383836 22171814 </pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 15:21, 13 August 2013
- Description: spore coat protein, cell wall hydrolase
| Gene name | cwlJ |
| Synonyms | ycbQ |
| Essential | no |
| Product | spore coat protein, cell wall hydrolase |
| Function | spore germination |
| Gene expression levels in SubtiExpress: cwlJ | |
| MW, pI | 16 kDa, 9.354 |
| Gene length, protein length | 426 bp, 142 aa |
| Immediate neighbours | ycbP, ycbR |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
cell wall/ other, sporulation proteins, germination
This gene is a member of the following regulons
SigE regulon, SigK regulon, SpoIIID regulon
The gene
Basic information
- Locus tag: BSU02600
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: cwlJ family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P42249
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
B Setlow, J Yu, Y-Q Li, P Setlow
Analysis of the germination kinetics of individual Bacillus subtilis spores treated with hydrogen peroxide or sodium hypochlorite.
Lett Appl Microbiol: 2013, 57(4);259-65
[PubMed:23746146]
[WorldCat.org]
[DOI]
(I p)
Yunfeng Li, Xuan Y Butzin, Andrew Davis, Barbara Setlow, George Korza, Fatma Işik Üstok, Graham Christie, Peter Setlow, Bing Hao
Activity and regulation of various forms of CwlJ, SleB, and YpeB proteins in degrading cortex peptidoglycan of spores of Bacillus species in vitro and during spore germination.
J Bacteriol: 2013, 195(11);2530-40
[PubMed:23543708]
[WorldCat.org]
[DOI]
(I p)
Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814]
[WorldCat.org]
[DOI]
(I p)
Xuan Yi, Peter Setlow
Studies of the commitment step in the germination of spores of bacillus species.
J Bacteriol: 2010, 192(13);3424-33
[PubMed:20435722]
[WorldCat.org]
[DOI]
(I p)
Alicia Monroe, Peter Setlow
Localization of the transglutaminase cross-linking sites in the Bacillus subtilis spore coat protein GerQ.
J Bacteriol: 2006, 188(21);7609-16
[PubMed:16936016]
[WorldCat.org]
[DOI]
(P p)
Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190]
[WorldCat.org]
[DOI]
(P p)
Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836]
[WorldCat.org]
[DOI]
(I p)
Katerina Ragkousi, Peter Setlow
Transglutaminase-mediated cross-linking of GerQ in the coats of Bacillus subtilis spores.
J Bacteriol: 2004, 186(17);5567-75
[PubMed:15317760]
[WorldCat.org]
[DOI]
(P p)
Haridasan Chirakkal, Michele O'Rourke, Abdelmadjid Atrih, Simon J Foster, Anne Moir
Analysis of spore cortex lytic enzymes and related proteins in Bacillus subtilis endospore germination.
Microbiology (Reading): 2002, 148(Pt 8);2383-2392
[PubMed:12177332]
[WorldCat.org]
[DOI]
(P p)
Irina Bagyan, Peter Setlow
Localization of the cortex lytic enzyme CwlJ in spores of Bacillus subtilis.
J Bacteriol: 2002, 184(4);1219-24
[PubMed:11807087]
[WorldCat.org]
[DOI]
(P p)
M Paidhungat, K Ragkousi, P Setlow
Genetic requirements for induction of germination of spores of Bacillus subtilis by Ca(2+)-dipicolinate.
J Bacteriol: 2001, 183(16);4886-93
[PubMed:11466292]
[WorldCat.org]
[DOI]
(P p)
S Ishikawa, K Yamane, J Sekiguchi
Regulation and characterization of a newly deduced cell wall hydrolase gene (cwlJ) which affects germination of Bacillus subtilis spores.
J Bacteriol: 1998, 180(6);1375-80
[PubMed:9515903]
[WorldCat.org]
[DOI]
(P p)
