CotM

• Description: spore coat protein (outer)
  
  

Gene name	cotM
Synonyms	yneL
Essential	no
Product	spore coat protein (outer)
Function	resistance of the spore
Gene expression levels in SubtiExpress: cotM
MW, pI	15 kDa, 3.942
Gene length, protein length	390 bp, 130 aa
Immediate neighbours	yneK, sspP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigE regulon, SigK regulon, GerE regulon

The gene

Basic information

• Locus tag: BSU17970

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • outer spore coat, localization depends on CotE PubMed

Database entries

• Structure:

• UniProt: Q45058

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cotM PubMed

• Expression browser: cotM PubMed

• Sigma factor: SigE PubMed, SigK PubMed

• Regulation:
  • expressed during sporulation in the mother cell (SigE, SigK, GerE) PubMed

• Regulatory mechanism:
  • GerE: transcription repression PubMed

• Additional information:
  • A ncRNA is predicted between yneK and cotM PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

S Reischl, S Thake, G Homuth, W Schumann
Transcriptional analysis of three Bacillus subtilis genes coding for proteins with the alpha-crystallin domain characteristic of small heat shock proteins.
FEMS Microbiol Lett: 2001, 194(1);99-103
[PubMed:11150673] [WorldCat.org] [DOI] (P p)

A O Henriques, B W Beall, C P Moran
CotM of Bacillus subtilis, a member of the alpha-crystallin family of stress proteins, is induced during development and participates in spore outer coat formation.
J Bacteriol: 1997, 179(6);1887-97
[PubMed:9068633] [WorldCat.org] [DOI] (P p)