Difference between revisions of "BshA"

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= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
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{{SubtiWiki regulon|[[Spx regulon]]}}
  
  
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
 +
** subject to feedback inhibition by bacillithiol {{PubMed|22569254}}
  
 
* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ypjH_2357078_2358211_-1 bshA] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ypjH_2357078_2358211_-1 bshA] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
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* '''[[Sigma factor]]:''' [[SigA]]  {{PubMed|23894131}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** induced by diamide stess (thiol depletion) ([[Spx]]) {{PubMed|23894131}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 +
** [[Spx]]: transcription activation {{PubMed|23894131}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:''' ''bshA::mls'' available in [[John_Helmann]] lab  
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* '''Mutant:'''  
** GP88 (''ypjH''::''pX2''(''cat'')), available in [[Stülke]] lab
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** ''bshA::mls'' available in [[John_Helmann]] lab  
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** GP88 (''bshA''::''pX2''(''cat'')), available in [[Jörg Stülke]]'s lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
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=References=
 
=References=
'''Additional references: ''' {{PubMed|20799687}}
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<pubmed> 20308541 19578333 22569254 20799687 23894131</pubmed>
<pubmed> 20308541 19578333 22569254 </pubmed>
 
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 12:13, 1 August 2013

  • Description: L-malic acid glycosyltransferase, involved in bacillithiol synthesis

Gene name bshA
Synonyms jojH, ypjH
Essential no
Product L-malic acid glycosyltransferase
Function biosynthesis of bacillithiol
Gene expression levels in SubtiExpress: bshA
MW, pI 41 kDa, 6.149
Gene length, protein length 1131 bp, 377 aa
Immediate neighbours cca, bshB1
Sequences Protein DNA DNA_with_flanks
Genetic context
YpjH context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BshA expression.png















Categories containing this gene/protein

miscellaneous metabolic pathways, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

Spx regulon


The gene

Basic information

  • Locus tag: BSU22460

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: UDP-GlcNAc + L-malate = GlcNAc(α1→2)L-malate PubMed; also uses D-malate as a substrate, but with much lower affinity PubMed
  • Protein family: NamA subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • subject to feedback inhibition by bacillithiol PubMed

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced by diamide stess (thiol depletion) (Spx) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References