Difference between revisions of "BshB1"
| Line 1: | Line 1: | ||
| − | * '''Description:''' N-acetylglucosamin-malate deacetylase, involved in bacillithiol synthesis <br/><br/> | + | * '''Description:''' N-acetylglucosamin-malate deacetylase, involved in bacillithiol synthesis (major enzyme)<br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
| Line 37: | Line 37: | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
<br/><br/><br/><br/> | <br/><br/><br/><br/> | ||
| − | + | <br/><br/> | |
| − | |||
| − | |||
| − | |||
| − | |||
= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| Line 48: | Line 44: | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| + | {{SubtiWiki regulon|[[Spx regulon]]}} | ||
| Line 113: | Line 110: | ||
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ypjG_2358208_2358918_-1 bshB1] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ypjG_2358208_2358918_-1 bshB1] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|23894131}} |
* '''Regulation:''' | * '''Regulation:''' | ||
| + | ** induced by diamide stess (thiol depletion) ([[Spx]]) {{PubMed|23894131}} | ||
* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
| + | ** [[Spx]]: transcription activation {{PubMed|23894131}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
| Line 141: | Line 140: | ||
=References= | =References= | ||
| − | <pubmed> 20308541 19578333 </pubmed> | + | <pubmed> 20308541 19578333 23894131</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 12:13, 1 August 2013
- Description: N-acetylglucosamin-malate deacetylase, involved in bacillithiol synthesis (major enzyme)
| Gene name | bshB1 |
| Synonyms | jojG, ypjG |
| Essential | no |
| Product | N-acetylglucosamin-malate deacetylase |
| Function | biosynthesis of bacillithiol |
| Gene expression levels in SubtiExpress: bshB1 | |
| Metabolic function and regulation of this protein in SubtiPathways: Lys, Thr | |
| MW, pI | 24 kDa, 6.343 |
| Gene length, protein length | 672 bp, 224 aa |
| Immediate neighbours | bshA, mgsA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
miscellaneous metabolic pathways, resistance against oxidative and electrophile stress
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU22470
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: GlcNAc-Mal -> GlcN-Mal + Ac
- Protein family:
- Paralogous protein(s): BshB2
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P42981
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant: bshB1 null mutant and bshB1 / bshB2 double null mutant available in John Helmann lab
- GP87 (ypjG::pX2(cat)), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
