ParB

• Description: chromosome positioning near the pole and transport through the polar septum / antagonist of Soj-dependent inhibition of sporulation initiation
  
  

Gene name	spo0J
Synonyms	spo0JB
Essential	no
Product	site-specific DNA-binding protein
Function	chromosome positioning before asymmetric septation, specifying its orientation, and imposing directionality on its subsequent transport through the septum; required for sporulation (antagonist of Soj-dependent inhibition of sporulation initiation)
Gene expression levels in SubtiExpress: spo0J
Interactions involving this protein in SubtInteract: Spo0J
MW, pI	32 kDa, 8.417
Gene length, protein length	846 bp, 282 aa
Immediate neighbours	yyaC, soj
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA condensation/ segregation, sporulation/ other

This gene is a member of the following regulons

Spo0A regulon

The gene

Basic information

• Locus tag: BSU40960

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: inhibits Soj dimerization and concomitant DNA-binding activity by stimulating Soj ATPase activity PubMed

• Protein family: parB family (according to Swiss-Prot)

• Paralogous protein(s): Noc

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: Spo0J-Soj PubMed

• Localization:
  • chromosome centromer PubMed

Database entries

• Structure:

• UniProt: P26497

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: soj-spo0J (according to DBTBS)

• Expression browser: spo0J PubMed

• Sigma factor:

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1S135 ( spo0J::spec), PubMed, available at BGSC
  • 1S136 ( spo0J::spec), PubMed, available at BGSC
  • 1S137 ( spo0J::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Rodrigo Reyes-Lamothe, Emilien Nicolas, David J Sherratt
Chromosome replication and segregation in bacteria.
Annu Rev Genet: 2012, 46;121-43
[PubMed:22934648] [WorldCat.org] [DOI] (I p)