Difference between revisions of "NagA"

From SubtiWiki
Jump to: navigation, search
(Expression and regulation)
Line 107: Line 107:
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nagA_3595356_3596546_1 nagA] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=nagA_3595356_3596546_1 nagA] {{PubMed|22383849}}
  
* '''Sigma factor:''' [[SigA]] {{PubMed|23667565}}  
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|23667565}}  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
Line 138: Line 138:
  
 
=References=
 
=References=
<pubmed>10627040, 14343123,12850135 6174502, 14557261 23667565 21602348</pubmed>
+
<pubmed>10627040, 14343123,12850135 6174502, 14557261 23667565 21602348 23876412</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 10:48, 25 July 2013

  • Description: N-acetylglucosamine-6-phosphate deacetylase

Gene name nagA
Synonyms
Essential no
Product N-acetylglucosamine-6-phosphate deacetylase
Function N-acetylglucosamine utilization
Gene expression levels in SubtiExpress: nagA
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism, Murein recycling
MW, pI 42 kDa, 5.276
Gene length, protein length 1188 bp, 396 aa
Immediate neighbours hprK, nagB
Sequences Protein DNA DNA_with_flanks
Genetic context
NagA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NagA expression.png















Categories containing this gene/protein

cell wall degradation/ turnover, utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, NagR regulon

The gene

Basic information

  • Locus tag: BSU35010

Phenotypes of a mutant

  • no growth on N-acetylglucosamine PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: N-acetyl-D-glucosamine 6-phosphate + H2O = D-glucosamine 6-phosphate + acetate (according to Swiss-Prot)
  • Protein family: nagA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: K(M): 1.4 mM PubMed
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]

Additional information

Expression and regulation

  • Regulation:
    • induced in the presence of N-acetylglucosamine (NagR) PubMed
    • repressed by glucose (2.9-fold) (CcpA) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Yanfeng Liu, Long Liu, Hyun-dong Shin, Rachel R Chen, Jianghua Li, Guocheng Du, Jian Chen
Pathway engineering of Bacillus subtilis for microbial production of N-acetylglucosamine.
Metab Eng: 2013, 19;107-15
[PubMed:23876412] [WorldCat.org] [DOI] (I p)

Isabelle Gaugué, Jacques Oberto, Harald Putzer, Jacqueline Plumbridge
The use of amino sugars by Bacillus subtilis: presence of a unique operon for the catabolism of glucosamine.
PLoS One: 2013, 8(5);e63025
[PubMed:23667565] [WorldCat.org] [DOI] (I e)

Ralph Bertram, Sébastien Rigali, Natalie Wood, Andrzej T Lulko, Oscar P Kuipers, Fritz Titgemeyer
Regulon of the N-acetylglucosamine utilization regulator NagR in Bacillus subtilis.
J Bacteriol: 2011, 193(14);3525-36
[PubMed:21602348] [WorldCat.org] [DOI] (I p)

Florence Vincent, David Yates, Elspeth Garman, Gideon J Davies, James A Brannigan
The three-dimensional structure of the N-acetylglucosamine-6-phosphate deacetylase, NagA, from Bacillus subtilis: a member of the urease superfamily.
J Biol Chem: 2004, 279(4);2809-16
[PubMed:14557261] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)

H L Mobley, R J Doyle, U N Streips, S O Langemeier
Transport and incorporation of N-acetyl-D-glucosamine in Bacillus subtilis.
J Bacteriol: 1982, 150(1);8-15
[PubMed:6174502] [WorldCat.org] [DOI] (P p)

C J BATES, C A PASTERNAK
FURTHER STUDIES ON THE REGULATION OF AMINO SUGAR METABOLISM IN BACILLUS SUBTILIS.
Biochem J: 1965, 96(1);147-54
[PubMed:14343123] [WorldCat.org] [DOI] (P p)