Difference between revisions of "ThiT"

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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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=== Database entries ===
 
=== Database entries ===
  
* '''Structure:'''
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* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3RLB 3RLB] (from ''Lactococcus lactis'', 32% identity) {{PubMed|21706007}}
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O32074 O32074]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/O32074 O32074]
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=thiT_3179306_3179884_1 thiT] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=thiT_3179306_3179884_1 thiT] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
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* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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=References=
 
=References=
  
<pubmed>16291685,,18763711, 12376536</pubmed>
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<pubmed>16291685, 21706007,18763711, 12376536</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 14:25, 12 July 2013

  • Description: thiamine permease

Gene name thiT
Synonyms yuaJ
Essential no
Product thiamine permease
Function thiamine uptake
Gene expression levels in SubtiExpress: thiT
Metabolic function and regulation of this protein in SubtiPathways:
Thiamin
MW, pI 20 kDa, 10.237
Gene length, protein length 576 bp, 192 aa
Immediate neighbours rrnB-16S, yuaI
Sequences Protein DNA DNA_with_flanks
Genetic context
YuaJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ThiT expression.png















Categories containing this gene/protein

transporters/ other, biosynthesis of cofactors, membrane proteins

This gene is a member of the following regulons

Thi-box

The gene

Basic information

  • Locus tag: BSU30990

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: proton-coupled thiamine transporter family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3RLB (from Lactococcus lactis, 32% identity) PubMed
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Guus B Erkens, Ronnie P-A Berntsson, Faizah Fulyani, Maria Majsnerowska, Andreja Vujičić-Žagar, Josy Ter Beek, Bert Poolman, Dirk Jan Slotboom
The structural basis of modularity in ECF-type ABC transporters.
Nat Struct Mol Biol: 2011, 18(7);755-60
[PubMed:21706007] [WorldCat.org] [DOI] (I e)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Ghislain Schyns, Sébastien Potot, Yi Geng, Teresa M Barbosa, Adriano Henriques, John B Perkins
Isolation and characterization of new thiamine-deregulated mutants of Bacillus subtilis.
J Bacteriol: 2005, 187(23);8127-36
[PubMed:16291685] [WorldCat.org] [DOI] (P p)

Dmitry A Rodionov, Alexey G Vitreschak, Andrey A Mironov, Mikhail S Gelfand
Comparative genomics of thiamin biosynthesis in procaryotes. New genes and regulatory mechanisms.
J Biol Chem: 2002, 277(50);48949-59
[PubMed:12376536] [WorldCat.org] [DOI] (P p)