Difference between revisions of "IspH"
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| − | * '''Description:''' | + | * '''Description:''' (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 7th step in the MEP pathway of isoprenoid biosynthesis <br/><br/> |
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|style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] | |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed] | ||
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase |
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|style="background:#ABCDEF;" align="center"|'''Function''' || MEP pathway of isoprenoid biosynthesis | |style="background:#ABCDEF;" align="center"|'''Function''' || MEP pathway of isoprenoid biosynthesis | ||
Revision as of 08:21, 9 July 2013
- Description: (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 7th step in the MEP pathway of isoprenoid biosynthesis
| Gene name | ispH |
| Synonyms | yqfP |
| Essential | yes PubMed |
| Product | (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase |
| Function | MEP pathway of isoprenoid biosynthesis |
| Gene expression levels in SubtiExpress: ispH | |
| MW, pI | 34 kDa, 5.615 |
| Gene length, protein length | 942 bp, 314 aa |
| Immediate neighbours | yqfQ, yqfO |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biosynthesis of lipids, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU25160
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H (according to Swiss-Prot)
- Protein family: ispH family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: P54473
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Tobias Gräwert, Felix Rohdich, Ingrid Span, Adelbert Bacher, Wolfgang Eisenreich, Jörg Eppinger, Michael Groll
Structure of active IspH enzyme from Escherichia coli provides mechanistic insights into substrate reduction.
Angew Chem Int Ed Engl: 2009, 48(31);5756-9
[PubMed:19569147]
[WorldCat.org]
[DOI]
(I p)
Mattijs K Julsing, Michael Rijpkema, Herman J Woerdenbag, Wim J Quax, Oliver Kayser
Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
Appl Microbiol Biotechnol: 2007, 75(6);1377-84
[PubMed:17458547]
[WorldCat.org]
[DOI]
(P p)
