AlsR

• Description: transcriptional activator of the alsS-alsD operon
  
  

Gene name	alsR
Synonyms
Essential	no
Product	transcriptional activator(LysR family)
Function	regulation of acetoin synthesis (alsS-alsD)
Gene expression levels in SubtiExpress: alsR
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	34 kDa, 7.288
Gene length, protein length	906 bp, 302 aa
Immediate neighbours	alsS, ywrK
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

carbon core metabolism, transcription factors and their control

The AlsR regulon:

• alsS-alsD

The gene

Basic information

• Locus tag: BSU36020

Phenotypes of a mutant

no acetoin production

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed
• Inactivation of alsR, sigD and sigW increases competitive fitness of Bacillus subtilis under non-sporulating conditions PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds the promoter region of the alsS-alsD operon in the presence of acetate, activates transcription

• Protein family: LysR family of transcription factors

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:
  • K(D) value for the binding site in the alsS promoter region: 33.5 nM PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: Q04778

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: alsR PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: AMBs3 (spc), available in the Stülke lab
  • 1A978 ( alsR::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Elisabeth Härtig, Braunschweig, Germany Homepage

References

Reviews

Elisabeth Härtig, Dieter Jahn
Regulation of the anaerobic metabolism in Bacillus subtilis.
Adv Microb Physiol: 2012, 61;195-216
[PubMed:23046954] [WorldCat.org] [DOI] (I p)