Difference between revisions of "AlaS"
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Revision as of 14:04, 16 May 2013
- Description: alanine-tRNA synthetase
| Gene name | alaS |
| Synonyms | |
| Essential | yes PubMed |
| Product | alanine-tRNA synthetase |
| Function | translation |
| Gene expression levels in SubtiExpress: alaS | |
| Metabolic function and regulation of this protein in SubtiPathways: tRNA charging | |
| MW, pI | 97 kDa, 4.922 |
| Gene length, protein length | 2634 bp, 878 aa |
| Immediate neighbours | yrzL, yrrI |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU27410
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala) (according to Swiss-Prot)
- Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure: 1YFS (enzyme from Aquifex aeolicus) PubMed, 3HXU (enzyme from E. coli with alanine) PubMed
- UniProt: O34526
- KEGG entry: [2]
- E.C. number: 6.1.1.7
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulatory mechanism:
- T-box: RNA switch, transcriptional antitermination PubMed
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Min Guo, Yeeting E Chong, Ryan Shapiro, Kirk Beebe, Xiang-Lei Yang, Paul Schimmel
Paradox of mistranslation of serine for alanine caused by AlaRS recognition dilemma.
Nature: 2009, 462(7274);808-12
[PubMed:20010690]
[WorldCat.org]
[DOI]
(I p)
Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532]
[WorldCat.org]
[DOI]
(I p)
Manal A Swairjo, Paul R Schimmel
Breaking sieve for steric exclusion of a noncognate amino acid from active site of a tRNA synthetase.
Proc Natl Acad Sci U S A: 2005, 102(4);988-93
[PubMed:15657145]
[WorldCat.org]
[DOI]
(P p)
