PyrC

• Description: dihydroorotase
  
  

Gene name	pyrC
Synonyms
Essential	no
Product	dihydroorotase
Function	pyrimidine biosynthesis
Gene expression levels in SubtiExpress: pyrC
Metabolic function and regulation of this protein in SubtiPathways: Pyrimidines, Nucleotides (regulation)
MW, pI	46 kDa, 5.427
Gene length, protein length	1284 bp, 428 aa
Immediate neighbours	pyrB, pyrAA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides

This gene is a member of the following regulons

PyrR regulon

The gene

Basic information

• Locus tag: BSU15500

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-dihydroorotate + H₂O = N-carbamoyl-L-aspartate (according to Swiss-Prot)

• Protein family: Type 2 subfamily (according to Swiss-Prot)

• Paralogous protein(s): PucH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3GRI (from Staphylococcus aureus subsp. aureus mw2, 60% identity, 72% similarity)

• UniProt: P25995

• KEGG entry: [3]

• E.C. number: 3.5.2.3

Expression and regulation

• Operon: pyrR-pyrP-pyrB-pyrC-pyrAA-pyrAB-pyrK-pyrD-pyrF-pyrE PubMed

• Expression browser: pyrC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced in the absence of uridine nucleotides (PyrR) PubMed

• Regulatory mechanism:
  • PyrR: RNA switch, transcription termination/ antitermination (in the presence of uridine nucleotides: termination, in their absence: antitermination) PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: