Difference between revisions of "CsrA"

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2,  http://nomorealcoholism.info/ antabuse,
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* '''Description:''' motility regulator, binds to the ''[[hag]]'' mRNA to inhibit its translation <br/><br/>
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{| align="right" border="1" cellpadding="2"
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|-
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|style="background:#ABCDEF;" align="center"|'''Gene name'''
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|''csrA''
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|-
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yviG ''
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|-
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no
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|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || motility regulator
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|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || control of ''[[hag]]'' translation
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35370 csrA]
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|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CsrA CsrA]
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|-
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 8 kDa, 6.09 
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|-
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 222 bp, 74 aa
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|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[hag]]'', ''[[fliW]]''
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|-
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU35370 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU35370 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU35370 DNA_with_flanks]
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|-
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|colspan="2" | '''Genetic context''' <br/> [[Image:csrA_context.gif]]
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  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|-
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=csrA_3636046_3636270_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:csrA_expression.png|500px]]
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|-
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|}
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__TOC__
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<br/><br/><br/><br/>
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<br/><br/><br/><br/>
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<br/><br/><br/><br/>
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<br/><br/><br/><br/>
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<br/><br/>
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= [[Categories]] containing this gene/protein =
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{{SubtiWiki category|[[motility and chemotaxis]]}}
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= This gene is a member of the following [[regulons]] =
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=The [[CsrA regulon]]: ''[[hag]]''=
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=The gene=
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=== Basic information ===
 +
 
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* '''Locus tag:''' BSU35370
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===Phenotypes of a mutant ===
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=== Database entries ===
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/csrA.html]
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* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10654]
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=== Additional information===
 +
 
 +
 
 +
=The protein=
 +
 
 +
=== Basic information/ Evolution ===
 +
 
 +
* '''Catalyzed reaction/ biological activity:'''
 +
** [[CsrA]] binds the ''[[hag]]'' mRNA to inhibit translation {{PubMed|21895793}}
 +
 
 +
* '''Protein family:''' csrA family (according to Swiss-Prot)
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* '''Paralogous protein(s):'''
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 +
=== Extended information on the protein ===
 +
 
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* '''Kinetic information:'''
 +
 
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* '''Domains:'''
 +
 
 +
* '''Modification:'''
 +
 
 +
* '''Cofactor(s):'''
 +
 
 +
* '''Effectors of protein activity:'''
 +
** [[CsrA]] is sequestered by interaction with [[FliW]] (the two proteins form a conserved module in many bacteria) {{PubMed|21895793}}
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* '''[[SubtInteract|Interactions]]:'''
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** [[FliW]]-[[CsrA]] {{PubMed|21895793}}
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* '''[[Localization]]:'''
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=== Database entries ===
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* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1T3O 1T3O]
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* '''UniProt:''' [http://www.uniprot.org/uniprot/P33911 P33911]
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* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU35370]
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* '''E.C. number:'''
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=== Additional information===
 +
* [[CsrA]] and [[FliW]] form a conserved module in many bacteria {{PubMed|21895793}}
 +
 
 +
=Expression and regulation=
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* '''Operon:''' ''csrA'' {{PubMed|8969505}}
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=csrA_3636046_3636270_-1 csrA] {{PubMed|22383849}}
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* '''[[Sigma factor]]:'''
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* '''Regulation:'''
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* '''Regulatory mechanism:'''
 +
 
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* '''Additional information:'''
 +
 
 +
=Biological materials =
 +
 
 +
* '''Mutant:''' GP469 (spc), available in [[Stülke]] lab
 +
 
 +
* '''Expression vector:'''
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** pGP381 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab
 +
** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP383, available in [[Stülke]] lab
 +
     
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* '''lacZ fusion:''' pGP461 (in [[pAC7]]), available in [[Stülke]] lab
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* '''GFP fusion:'''
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* '''two-hybrid system:'''
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* '''Antibody:'''
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=Labs working on this gene/protein=
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=Your additional remarks=
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=References=
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==Reviews==
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'''Additional Reviews:''' {{PubMed|22672726}}
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<pubmed>19385727</pubmed>
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==Original Publications==
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<pubmed>16822857,17555441 8969505, 21895793 23144244</pubmed>
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[[Category:Protein-coding genes]]

Revision as of 07:08, 15 May 2013

  • Description: motility regulator, binds to the hag mRNA to inhibit its translation

Gene name csrA
Synonyms yviG
Essential no
Product motility regulator
Function control of hag translation
Gene expression levels in SubtiExpress: csrA
Interactions involving this protein in SubtInteract: CsrA
MW, pI 8 kDa, 6.09
Gene length, protein length 222 bp, 74 aa
Immediate neighbours hag, fliW
Sequences Protein DNA DNA_with_flanks
Genetic context
CsrA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CsrA expression.png



















Categories containing this gene/protein

motility and chemotaxis

This gene is a member of the following regulons

The CsrA regulon: hag

The gene

Basic information

  • Locus tag: BSU35370

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: csrA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • CsrA is sequestered by interaction with FliW (the two proteins form a conserved module in many bacteria) PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP469 (spc), available in Stülke lab
  • Expression vector:
    • pGP381 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
    • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP383, available in Stülke lab
  • lacZ fusion: pGP461 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Additional Reviews: PubMed

Paul Babitzke, Carol S Baker, Tony Romeo
Regulation of translation initiation by RNA binding proteins.
Annu Rev Microbiol: 2009, 63;27-44
[PubMed:19385727] [WorldCat.org] [DOI] (I p)

Original Publications

Sampriti Mukherjee, Paul Babitzke, Daniel B Kearns
FliW and FliS function independently to control cytoplasmic flagellin levels in Bacillus subtilis.
J Bacteriol: 2013, 195(2);297-306
[PubMed:23144244] [WorldCat.org] [DOI] (I p)

Sampriti Mukherjee, Helen Yakhnin, Dave Kysela, Josh Sokoloski, Paul Babitzke, Daniel B Kearns
CsrA-FliW interaction governs flagellin homeostasis and a checkpoint on flagellar morphogenesis in Bacillus subtilis.
Mol Microbiol: 2011, 82(2);447-61
[PubMed:21895793] [WorldCat.org] [DOI] (I p)

Helen Yakhnin, Pallavi Pandit, Tom J Petty, Carol S Baker, Tony Romeo, Paul Babitzke
CsrA of Bacillus subtilis regulates translation initiation of the gene encoding the flagellin protein (hag) by blocking ribosome binding.
Mol Microbiol: 2007, 64(6);1605-20
[PubMed:17555441] [WorldCat.org] [DOI] (P p)

Prajna R Kulkarni, Xiaohui Cui, Joshua W Williams, Ann M Stevens, Rahul V Kulkarni
Prediction of CsrA-regulating small RNAs in bacteria and their experimental verification in Vibrio fischeri.
Nucleic Acids Res: 2006, 34(11);3361-9
[PubMed:16822857] [WorldCat.org] [DOI] (I e)

B Soldo, V Lazarevic, C Mauël, D Karamata
Sequence of the 305 degrees-307 degrees region of the Bacillus subtilis chromosome.
Microbiology (Reading): 1996, 142 ( Pt 11);3079-88
[PubMed:8969505] [WorldCat.org] [DOI] (P p)

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