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| − | * '''Description:''' motility regulator, binds to the ''[[hag]]'' mRNA to inhibit its translation <br/><br/>
| + | 0, http://acne-trouble.info/#1758 accutane, |
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| − | {| align="right" border="1" cellpadding="2"
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| − | |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| − | |''csrA''
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| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yviG ''
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| − | |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || motility regulator
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| − | |style="background:#ABCDEF;" align="center"|'''Function''' || control of ''[[hag]]'' translation
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU35370 csrA]
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CsrA CsrA]
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| − | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 8 kDa, 6.09
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| − | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 222 bp, 74 aa
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| − | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[hag]]'', ''[[fliW]]''
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| − | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU35370 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU35370 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU35370 DNA_with_flanks]
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| − | |colspan="2" | '''Genetic context''' <br/> [[Image:csrA_context.gif]]
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| − | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| − | |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=csrA_3636046_3636270_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:csrA_expression.png|500px]]
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| − | __TOC__
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| − | <br/><br/><br/><br/>
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| − | <br/><br/><br/><br/>
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| − | <br/><br/><br/><br/>
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| − | <br/><br/>
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| − | = [[Categories]] containing this gene/protein =
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| − | {{SubtiWiki category|[[motility and chemotaxis]]}}
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| − | = This gene is a member of the following [[regulons]] =
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| − | =The [[CsrA regulon]]: ''[[hag]]''=
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| − | =The gene=
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| − | === Basic information ===
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| − | * '''Locus tag:''' BSU35370
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| − | ===Phenotypes of a mutant ===
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| − | === Database entries ===
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| − | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/csrA.html]
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| − | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10654]
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| − | === Additional information===
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| − | =The protein=
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| − | === Basic information/ Evolution ===
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| − | * '''Catalyzed reaction/ biological activity:'''
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| − | ** [[CsrA]] binds the ''[[hag]]'' mRNA to inhibit translation {{PubMed|21895793}}
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| − | * '''Protein family:''' csrA family (according to Swiss-Prot)
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| − | * '''Paralogous protein(s):'''
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| − | === Extended information on the protein ===
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| − | * '''Kinetic information:'''
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| − | * '''Domains:'''
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| − | * '''Modification:'''
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| − | * '''Cofactor(s):'''
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| − | * '''Effectors of protein activity:'''
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| − | ** [[CsrA]] is sequestered by interaction with [[FliW]] (the two proteins form a conserved module in many bacteria) {{PubMed|21895793}}
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| − | * '''[[SubtInteract|Interactions]]:'''
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| − | ** [[FliW]]-[[CsrA]] {{PubMed|21895793}}
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| − | * '''[[Localization]]:'''
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| − | === Database entries ===
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| − | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1T3O 1T3O]
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| − | * '''UniProt:''' [http://www.uniprot.org/uniprot/P33911 P33911]
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| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU35370]
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| − | * '''E.C. number:'''
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| − | === Additional information===
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| − | * [[CsrA]] and [[FliW]] form a conserved module in many bacteria {{PubMed|21895793}}
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| − | =Expression and regulation=
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| − | * '''Operon:''' ''csrA'' {{PubMed|8969505}}
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| − | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=csrA_3636046_3636270_-1 csrA] {{PubMed|22383849}}
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| − | * '''[[Sigma factor]]:'''
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| − | * '''Regulation:'''
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| − | * '''Regulatory mechanism:'''
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| − | * '''Additional information:'''
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| − | =Biological materials =
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| − | * '''Mutant:''' GP469 (spc), available in [[Stülke]] lab
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| − | * '''Expression vector:'''
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| − | ** pGP381 (N-terminal Strep-tag, purification from ''B. subtilis'', for [[SPINE]], in [[pGP380]]), available in [[Stülke]] lab
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| − | ** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP383, available in [[Stülke]] lab
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| − | * '''lacZ fusion:''' pGP461 (in [[pAC7]]), available in [[Stülke]] lab
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| − | * '''GFP fusion:'''
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| − | * '''two-hybrid system:'''
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| − | * '''Antibody:'''
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| − | =Labs working on this gene/protein=
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| − | =Your additional remarks=
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| − | =References=
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| − | ==Reviews==
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| − | '''Additional Reviews:''' {{PubMed|22672726}}
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| − | <pubmed>19385727</pubmed>
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| − | ==Original Publications==
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| − | <pubmed>16822857,17555441 8969505, 21895793 23144244</pubmed>
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| − | [[Category:Protein-coding genes]]
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