DltE

• Description: D-alanine transfer from undecaprenol-phosphate to the poly(glycerophosphate) chain, alanylation of teichoic acid provides some resistance against positively charged antimicrobial peptides
  
  

Gene name	dltE
Synonyms	ipa-1r
Essential	no
Product	D-alanine transfer from undecaprenol-phosphate to the poly(glycerophosphate) chain
Function	biosynthesis of teichoic acid
Gene expression levels in SubtiExpress: dltE
MW, pI	28 kDa, 5.095
Gene length, protein length	756 bp, 252 aa
Immediate neighbours	dltD, ywaA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, cell envelope stress proteins (controlled by SigM, V, W, X, Y)

This gene is a member of the following regulons

SigD regulon, SigM regulon, SigX regulon, Spo0A regulon, stringent response, YvrHb regulon

The gene

Basic information

• Locus tag: BSU38540

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: short-chain dehydrogenases/reductases (SDR) family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P39577

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dltA-dltB-dltC-dltD-dltE PubMed

• Expression browser: dltE PubMed

• Sigma factor: SigD PubMed, SigM PubMed, SigX PubMed

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed
  • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • expression is reduced in a SigV mutant PubMed

• Regulatory mechanism:
  • activated by YvrHb PubMed
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Min Cao, John D Helmann
The Bacillus subtilis extracytoplasmic-function sigmaX factor regulates modification of the cell envelope and resistance to cationic antimicrobial peptides.
J Bacteriol: 2004, 186(4);1136-46
[PubMed:14762009] [WorldCat.org] [DOI] (P p)

M Perego, P Glaser, A Minutello, M A Strauch, K Leopold, W Fischer
Incorporation of D-alanine into lipoteichoic acid and wall teichoic acid in Bacillus subtilis. Identification of genes and regulation.
J Biol Chem: 1995, 270(26);15598-606
[PubMed:7797557] [WorldCat.org] [DOI] (P p)