PucM

• Description: uricase
  
  

Gene name	pucM
Synonyms	yunM
Essential	no
Product	uricase
Function	purine utilization
Gene expression levels in SubtiExpress: pucM
Metabolic function and regulation of this protein in SubtiPathways: Purine catabolism, Nucleotides (regulation)
MW, pI	13 kDa, 5.082
Gene length, protein length	363 bp, 121 aa
Immediate neighbours	pucL, yuzJ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of nucleotides

This gene is a member of the following regulons

PucR regulon, TnrA regulon

The gene

Basic information

• Locus tag: BSU32460

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5-hydroxyisourate + H₂O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate (according to Swiss-Prot)

• Protein family: 5-hydroxyisourate hydrolase subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2H0E

• UniProt: O32142

• KEGG entry: [3]

• E.C. number: 3.5.2.17

Expression and regulation

• Operon:
  • pucR-pucJ-pucK-pucL-pucM PubMed
  • pucJ-pucK-pucL-pucM PubMed

• Expression browser: pucM PubMed

• Sigma factor:
  • pucJ: SigA PubMed

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed
  • PucR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: