MetK

• Description: S-adenosylmethionine synthetase
  
  

Gene name	metK
Synonyms	metE
Essential	yes PubMed
Product	S-adenosylmethionine synthetase
Function	S-adenosylmethionine synthesis
Gene expression levels in SubtiExpress: metK
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	43 kDa, 5.025
Gene length, protein length	1200 bp, 400 aa
Immediate neighbours	asnB, pckA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, essential genes

This gene is a member of the following regulons

S-box

The gene

Basic information

• Locus tag: BSU30550

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-methionine + H₂O = phosphate + diphosphate + S-adenosyl-L-methionine (according to Swiss-Prot)

• Protein family: AdoMet synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1FUG (from Escherichia coli, 63% identity, 75% similarity) PubMed

• UniProt: P54419

• KEGG entry: [3]

• E.C. number: 2.5.1.6

Expression and regulation

• Operon: metK PubMed

• Expression browser: metK PubMed

• Sigma factor:

• Regulation:
  • induced by methionine starvation (S-box) PubMed

• Regulatory mechanism:
  • S-box: riboswitch, binds S-adenosylmethionine resulting in transcription termination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion: pGP432 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Jerneja Tomsic, Brooke A McDaniel, Frank J Grundy, Tina M Henkin
Natural variability in S-adenosylmethionine (SAM)-dependent riboswitches: S-box elements in bacillus subtilis exhibit differential sensitivity to SAM In vivo and in vitro.
J Bacteriol: 2008, 190(3);823-33
[PubMed:18039762] [WorldCat.org] [DOI] (I p)

Brooke A McDaniel, Frank J Grundy, Vineeta P Kurlekar, Jerneja Tomsic, Tina M Henkin
Identification of a mutation in the Bacillus subtilis S-adenosylmethionine synthetase gene that results in derepression of S-box gene expression.
J Bacteriol: 2006, 188(10);3674-81
[PubMed:16672621] [WorldCat.org] [DOI] (P p)

F J Grundy, T M Henkin
The S box regulon: a new global transcription termination control system for methionine and cysteine biosynthesis genes in gram-positive bacteria.
Mol Microbiol: 1998, 30(4);737-49
[PubMed:10094622] [WorldCat.org] [DOI] (P p)

Alia Lapidus, Nathalie Galleron, Alexei Sorokin, S Dusko Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading): 1997, 143 ( Pt 11);3431-3441
[PubMed:9387221] [WorldCat.org] [DOI] (P p)