SpoIVCA

• Description: site-specific DNA recombinase required for creating the sigK gene (excision of the skin element)
  
  

Gene name	spoIVCA
Synonyms	cisA
Essential	no
Product	site-specific DNA recombinase
Function	excision of the skin element, creation of the sigK gene
Gene expression levels in SubtiExpress: spoIVCA
MW, pI	57 kDa, 9.498
Gene length, protein length	1500 bp, 500 aa
Immediate neighbours	spoIVCB, arsC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, sigma factors and their control, sporulation proteins, Skin element

This gene is a member of the following regulons

SigE regulon, SpoIIID regulon

The gene

Basic information

• Locus tag: BSU25770

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P17867

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoIVCA PubMed

• Expression browser: spoIVCA PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed

• Regulatory mechanism:
  • SpoIIID: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

R Halberg, L Kroos
Sporulation regulatory protein SpoIIID from Bacillus subtilis activates and represses transcription by both mother-cell-specific forms of RNA polymerase.
J Mol Biol: 1994, 243(3);425-36
[PubMed:7966271] [WorldCat.org] [DOI] (P p)

B Kunkel, R Losick, P Stragier
The Bacillus subtilis gene for the development transcription factor sigma K is generated by excision of a dispensable DNA element containing a sporulation recombinase gene.
Genes Dev: 1990, 4(4);525-35
[PubMed:2163341] [WorldCat.org] [DOI] (P p)

T Sato, Y Samori, Y Kobayashi
The cisA cistron of Bacillus subtilis sporulation gene spoIVC encodes a protein homologous to a site-specific recombinase.
J Bacteriol: 1990, 172(2);1092-8
[PubMed:2105293] [WorldCat.org] [DOI] (P p)

B Kunkel, K Sandman, S Panzer, P Youngman, R Losick
The promoter for a sporulation gene in the spoIVC locus of Bacillus subtilis and its use in studies of temporal and spatial control of gene expression.
J Bacteriol: 1988, 170(8);3513-22
[PubMed:2841290] [WorldCat.org] [DOI] (P p)