FabI

• Description: enoyl-acyl carrier protein reductase
  
  

Gene name	fabI
Synonyms	yjbW
Essential	no
Product	enoyl-acyl carrier protein reductase
Function	fatty acid biosynthesis
Gene expression levels in SubtiExpress: fabI
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis
MW, pI	27 kDa, 5.605
Gene length, protein length	774 bp, 258 aa
Immediate neighbours	thiD, cotO
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of lipids

This gene is a member of the following regulons

FapR regulon

The gene

Basic information

• Locus tag: BSU11720

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Acyl-[acyl-carrier-protein] + NAD⁺ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH (according to Swiss-Prot)

• Protein family: FabI subfamily (according to Swiss-Prot)

• Paralogous protein(s): FabL, one of the two proteins has to be present for viability PubMed

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • inhibited by triclosan PubMed

• Interactions:

• Localization:

Database entries

• Structure: 3OIF (complex with NAD and inhibitor triclosan) PubMed

• UniProt: P54616

• KEGG entry: [3]

• E.C. number: 1.3.1.9

Expression and regulation

• Operon: fabI PubMed

• Expression browser: fabI PubMed

• Sigma factor:

• Regulation:
  • expressed when the cells experience a lack of malonyl-CoA (FapR) PubMed
  • induced upon fatty acid biosynthesis inhibition PubMed

• Regulatory mechanism:
  • FapR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Yasutaro Fujita, Hiroshi Matsuoka, Kazutake Hirooka
Regulation of fatty acid metabolism in bacteria.
Mol Microbiol: 2007, 66(4);829-39
[PubMed:17919287] [WorldCat.org] [DOI] (P p)

Stephen W White, Jie Zheng, Yong-Mei Zhang, Rock
The structural biology of type II fatty acid biosynthesis.
Annu Rev Biochem: 2005, 74;791-831
[PubMed:15952903] [WorldCat.org] [DOI] (P p)