Difference between revisions of "YfiT"

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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yfiS]]'', ''[[yfiU]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yfiS]]'', ''[[yfiU]]''
 
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU08390 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU08390 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU08390 Advanced_DNA]
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU08390 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU08390 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU08390 DNA_with_flanks]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:yfiT_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yfiT_context.gif]]

Revision as of 09:44, 14 May 2013

  • Description: bacillithiol S-transferase

Gene name yfiT
Synonyms
Essential no
Product bacillithiol S-transferase
Function detoxification
Gene expression levels in SubtiExpress: yfiT
MW, pI 20 kDa, 6.612
Gene length, protein length 534 bp, 178 aa
Immediate neighbours yfiS, yfiU
Sequences Protein DNA DNA_with_flanks
Genetic context
YfiT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YfiT expression.png
























Categories containing this gene/protein

resistance against oxidative and electrophile stress

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU08390

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: metal hydrolase yfiT family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: yfiT (according to DBTBS)
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Gerald L Newton, Stephan S Leung, Judy I Wakabayashi, Mamta Rawat, Robert C Fahey
The DinB superfamily includes novel mycothiol, bacillithiol, and glutathione S-transferases.
Biochemistry: 2011, 50(49);10751-60
[PubMed:22059487] [WorldCat.org] [DOI] (I p)

Shyamala S Rajan, Xiaojing Yang, Ludmilla Shuvalova, Frank Collart, Wayne F Anderson
YfiT from Bacillus subtilis is a probable metal-dependent hydrolase with an unusual four-helix bundle topology.
Biochemistry: 2004, 43(49);15472-9
[PubMed:15581359] [WorldCat.org] [DOI] (P p)