Difference between revisions of "BglS"

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|style="background:#ABCDEF;" align="center"|'''Function''' || lichenan degradation
 
|style="background:#ABCDEF;" align="center"|'''Function''' || lichenan degradation
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU39070 bglS]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU39070 bglS]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[citH]]'', ''[[licT]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[citH]]'', ''[[licT]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15943&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU39070 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU39070 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU39070 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:bglS_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:bglS_context.gif]]

Revision as of 14:13, 13 May 2013

  • Description: endo-beta-1,3-1,4 glucanase

Gene name bglS
Synonyms bgl, licS
Essential no
Product endo-beta-1,3-1,4 glucanase
Function lichenan degradation
Gene expression levels in SubtiExpress: bglS
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism
MW, pI 27 kDa, 6.482
Gene length, protein length 726 bp, 242 aa
Immediate neighbours citH, licT
Sequences Protein DNA Advanced_DNA
Genetic context
BglS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BglS expression.png















Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, LicT regulon

The gene

Basic information

  • Locus tag: BSU39070

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 16 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed by glucose (3.1-fold) (CcpA) PubMed
    • expressed in the stationary phase (temporal activation) PubMed
    • weak induction by ß-glucosides (LicT) PubMed
  • Additional information:

Biological materials

  • Mutant: GP427 (licT-bglS, erm), BGW7 (cat), both available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References