Difference between revisions of "ThiE"

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|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of thiamine <br/>by 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate <br/>to yield thiamine phosphate
 
|style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of thiamine <br/>by 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate <br/>to yield thiamine phosphate
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU38290 thiE]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU38290 thiE]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/thiamin.html Thiamin]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/thiamin.html Thiamin]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywbL]]'', ''[[thiM]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywbL]]'', ''[[thiM]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15855&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU38290 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU38290 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU38290 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:thiE_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:thiE_context.gif]]

Revision as of 14:10, 13 May 2013

  • Description: thiamine-phosphate pyrophosphorylase

Gene name thiE
Synonyms thiC, ipa-26d, ywbK
Essential no
Product thiamine-phosphate pyrophosphorylase
Function biosynthesis of thiamine
by 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate
to yield thiamine phosphate
Gene expression levels in SubtiExpress: thiE
Metabolic function and regulation of this protein in SubtiPathways:
Thiamin
MW, pI 23 kDa, 5.058
Gene length, protein length 666 bp, 222 aa
Immediate neighbours ywbL, thiM
Sequences Protein DNA Advanced_DNA
Genetic context
ThiE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ThiE expression.png






























Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU38290

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate (according to Swiss-Prot)
  • Protein family: TMP-PPase family (according to Swiss-Prot)
  • Paralogous protein(s): TenI

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications

Y Zhang, S V Taylor, H J Chiu, T P Begley
Characterization of the Bacillus subtilis thiC operon involved in thiamine biosynthesis.
J Bacteriol: 1997, 179(9);3030-5
[PubMed:9139923] [WorldCat.org] [DOI] (P p)