Difference between revisions of "RapF"
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|style="background:#ABCDEF;" align="center"|'''Function''' || control of [[ComA]] activity | |style="background:#ABCDEF;" align="center"|'''Function''' || control of [[ComA]] activity | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU37460 rapF] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/RapF RapF] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/RapF RapF] | ||
| Line 24: | Line 24: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywhK]]'', ''[[phrF]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywhK]]'', ''[[phrF]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU37460 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU37460 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU37460 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:rapF_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:rapF_context.gif]] | ||
Revision as of 14:07, 13 May 2013
- Description: response regulator aspartate phosphatase, anti-activator protein, controls ComA activity
| Gene name | rapF |
| Synonyms | ywhJ |
| Essential | no |
| Product | response regulator aspartate phosphatase |
| Function | control of ComA activity |
| Gene expression levels in SubtiExpress: rapF | |
| Interactions involving this protein in SubtInteract: RapF | |
| MW, pI | 45 kDa, 5.18 |
| Gene length, protein length | 1143 bp, 381 aa |
| Immediate neighbours | ywhK, phrF |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
genetic competence, protein modification, transcription factors and their control, quorum sensing
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU37460
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Protein family: RAP family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity: binding of PhrF to RapF results in a conformational change of RapF and prevents it from binding ComA PubMed
Database entries
- Structure:
- UniProt: P71002
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Melinda D Baker, Matthew B Neiditch
Structural basis of response regulator inhibition by a bacterial anti-activator protein.
PLoS Biol: 2011, 9(12);e1001226
[PubMed:22215984]
[WorldCat.org]
[DOI]
(I p)
Natalia Comella, Alan D Grossman
Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis.
Mol Microbiol: 2005, 57(4);1159-74
[PubMed:16091051]
[WorldCat.org]
[DOI]
(P p)
Cristina Bongiorni, Shu Ishikawa, Sophie Stephenson, Naotake Ogasawara, Marta Perego
Synergistic regulation of competence development in Bacillus subtilis by two Rap-Phr systems.
J Bacteriol: 2005, 187(13);4353-61
[PubMed:15968044]
[WorldCat.org]
[DOI]
(P p)
Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135]
[WorldCat.org]
[DOI]
(P p)
R S McQuade, N Comella, A D Grossman
Control of a family of phosphatase regulatory genes (phr) by the alternate sigma factor sigma-H of Bacillus subtilis.
J Bacteriol: 2001, 183(16);4905-9
[PubMed:11466295]
[WorldCat.org]
[DOI]
(P p)
