Difference between revisions of "SmpB"
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|style="background:#ABCDEF;" align="center"|'''Function''' || trans-translation | |style="background:#ABCDEF;" align="center"|'''Function''' || trans-translation | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU33600 smpB] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 10.421 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 10.421 | ||
| Line 22: | Line 22: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ssrA]]'', ''[[rnr]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ssrA]]'', ''[[rnr]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU33600 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU33600 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU33600 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:smpB_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:smpB_context.gif]] | ||
Revision as of 13:51, 13 May 2013
- Description: tmRNA-binding protein
| Gene name | smpB |
| Synonyms | yvaI |
| Essential | no |
| Product | tmRNA-binding protein |
| Function | trans-translation |
| Gene expression levels in SubtiExpress: smpB | |
| MW, pI | 17 kDa, 10.421 |
| Gene length, protein length | 468 bp, 156 aa |
| Immediate neighbours | ssrA, rnr |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU33600
Phenotypes of a mutant
- impaired growth at high and low temperatures PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: smpB family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O32230
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
- Chet Price, Davis, USA homepage
Your additional remarks
References
Hiromi Ujiie, Tomoko Matsutani, Hisashi Tomatsu, Ai Fujihara, Chisato Ushida, Yasuhiko Miwa, Yasutaro Fujita, Hyouta Himeno, Akira Muto
Trans-translation is involved in the CcpA-dependent tagging and degradation of TreP in Bacillus subtilis.
J Biochem: 2009, 145(1);59-66
[PubMed:18977770]
[WorldCat.org]
[DOI]
(I p)
Ji-Hyun Shin, Chester W Price
The SsrA-SmpB ribosome rescue system is important for growth of Bacillus subtilis at low and high temperatures.
J Bacteriol: 2007, 189(10);3729-37
[PubMed:17369301]
[WorldCat.org]
[DOI]
(P p)
Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528]
[WorldCat.org]
[DOI]
(P p)
Jeffrey H Withey, David I Friedman
A salvage pathway for protein structures: tmRNA and trans-translation.
Annu Rev Microbiol: 2003, 57;101-23
[PubMed:12730326]
[WorldCat.org]
[DOI]
(P p)
T Wiegert, W Schumann
SsrA-mediated tagging in Bacillus subtilis.
J Bacteriol: 2001, 183(13);3885-9
[PubMed:11395451]
[WorldCat.org]
[DOI]
(P p)
A W Karzai, E D Roche, R T Sauer
The SsrA-SmpB system for protein tagging, directed degradation and ribosome rescue.
Nat Struct Biol: 2000, 7(6);449-55
[PubMed:10881189]
[WorldCat.org]
[DOI]
(P p)
