Difference between revisions of "SafA"
| Line 14: | Line 14: | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || spore coat formation | |style="background:#ABCDEF;" align="center"|'''Function''' || spore coat formation | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27840 safA] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SafA SafA] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SafA SafA] | ||
| Line 24: | Line 24: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[coxA]]'', ''[[nadA]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[coxA]]'', ''[[nadA]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU27840 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU27840 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU27840 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:safA_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:safA_context.gif]] | ||
Revision as of 13:28, 13 May 2013
- Description: morphogenetic protein associated with SpoVID, major organizer of the inner spore coat
| Gene name | safA |
| Synonyms | yrbA |
| Essential | no |
| Product | morphogenetic protein |
| Function | spore coat formation |
| Gene expression levels in SubtiExpress: safA | |
| Interactions involving this protein in SubtInteract: SafA | |
| MW, pI | 43 kDa, 5.753 |
| Gene length, protein length | 1161 bp, 387 aa |
| Immediate neighbours | coxA, nadA |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU27840
Phenotypes of a mutant
- mis-assembly of the inner spore coat PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: spore wall (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O32062
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
- Adriano Henriques, Lisbon, Portugal homepage
- Charles Moran, Emory University, NC, USA homepage
Your additional remarks
References
Additional publications: PubMed
Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814]
[WorldCat.org]
[DOI]
(I p)
Peter T McKenney, Adam Driks, Haig A Eskandarian, Paul Grabowski, Jonathan Guberman, Katherine H Wang, Zemer Gitai, Patrick Eichenberger
A distance-weighted interaction map reveals a previously uncharacterized layer of the Bacillus subtilis spore coat.
Curr Biol: 2010, 20(10);934-8
[PubMed:20451384]
[WorldCat.org]
[DOI]
(I p)
Teresa Costa, Anabela L Isidro, Charles P Moran, Adriano O Henriques
Interaction between coat morphogenetic proteins SafA and SpoVID.
J Bacteriol: 2006, 188(22);7731-41
[PubMed:16950916]
[WorldCat.org]
[DOI]
(P p)
A J Ozin, C S Samford, A O Henriques, C P Moran
SpoVID guides SafA to the spore coat in Bacillus subtilis.
J Bacteriol: 2001, 183(10);3041-9
[PubMed:11325931]
[WorldCat.org]
[DOI]
(P p)
A J Ozin, T Costa, A O Henriques, C P Moran
Alternative translation initiation produces a short form of a spore coat protein in Bacillus subtilis.
J Bacteriol: 2001, 183(6);2032-40
[PubMed:11222602]
[WorldCat.org]
[DOI]
(P p)
A J Ozin, A O Henriques, H Yi, C P Moran
Morphogenetic proteins SpoVID and SafA form a complex during assembly of the Bacillus subtilis spore coat.
J Bacteriol: 2000, 182(7);1828-33
[PubMed:10714986]
[WorldCat.org]
[DOI]
(P p)
H Takamatsu, T Kodama, T Nakayama, K Watabe
Characterization of the yrbA gene of Bacillus subtilis, involved in resistance and germination of spores.
J Bacteriol: 1999, 181(16);4986-94
[PubMed:10438771]
[WorldCat.org]
[DOI]
(P p)
