Difference between revisions of "CcpN"
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|style="background:#ABCDEF;" align="center"|'''Function''' || repressor of genes involved in <br/>gluconeogenesis (''[[gapB]]'','' [[pckA]]'') and of ''[[sr1]]'' | |style="background:#ABCDEF;" align="center"|'''Function''' || repressor of genes involved in <br/>gluconeogenesis (''[[gapB]]'','' [[pckA]]'') and of ''[[sr1]]'' | ||
|- | |- | ||
− | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25250 ccpN] |
|- | |- | ||
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CcpN CcpN] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CcpN CcpN] | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqfL]]'', ''[[glyS]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqfL]]'', ''[[glyS]]'' | ||
|- | |- | ||
− | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25250 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25250 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25250 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:yqzB_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:yqzB_context.gif]] |
Revision as of 13:20, 13 May 2013
- Description: transcriptional repressor of gluconeogenetic genes and of sr1. repression in the presence of glucose
Gene name | ccpN |
Synonyms | yqzB |
Essential | no |
Product | transcriptional regulator |
Function | repressor of genes involved in gluconeogenesis (gapB, pckA) and of sr1 |
Gene expression levels in SubtiExpress: ccpN | |
Interactions involving this protein in SubtInteract: CcpN | |
Metabolic function and regulation of this protein in SubtiPathways: Ammonium/ glutamate, Cys, Met & Sulfate assimilation, Central C-metabolism | |
MW, pI | 23.4 kDa, 7.22 |
Gene length, protein length | 636 bp, 212 amino acids |
Immediate neighbours | yqfL, glyS |
Sequences | Protein DNA Advanced_DNA |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
carbon core metabolism, transcription factors and their control, regulators of core metabolism
This gene is a member of the following regulons
The CcpN regulon:
The gene
Basic information
- Locus tag: BSU25250
Phenotypes of a mutant
Impaired growth on glucose due to re-routing of carbon from glycolysis to the pentose phosphate pathway PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: transcription repression of the gapB, pckA, and sr1 genes in the presence of glucose PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: O34994
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor:
- Regulation: constitutively expressed PubMed
- Additional information:
- the intracellular concentration of CcpN is about 4 myM (according to PubMed).
Biological materials
- Mutant:
- DB104 ccpN::cat, available in Sabine Brantl's lab
- GP1128 ccpN::cat, available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- Antibody: available in Sabine Brantl's lab
Labs working on this gene/protein
Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France
Sabine Brantl, Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany homepage
Uwe Sauer, ETH Zürich, Switzerland homepage
Your additional remarks
References
Reviews
Sabine Brantl, Andreas Licht
Characterisation of Bacillus subtilis transcriptional regulators involved in metabolic processes.
Curr Protein Pept Sci: 2010, 11(4);274-91
[PubMed:20408793]
[WorldCat.org]
[DOI]
(I p)
Original Publications
Nathalie Declerck, Catherine A Royer
Interactions in gene expression networks studied by two-photon fluorescence fluctuation spectroscopy.
Methods Enzymol: 2013, 519;203-30
[PubMed:23280112]
[WorldCat.org]
[DOI]
(I p)
Matthew L Ferguson, Dominique Le Coq, Matthieu Jules, Stéphane Aymerich, Ovidiu Radulescu, Nathalie Declerck, Catherine A Royer
Reconciling molecular regulatory mechanisms with noise patterns of bacterial metabolic promoters in induced and repressed states.
Proc Natl Acad Sci U S A: 2012, 109(1);155-60
[PubMed:22190493]
[WorldCat.org]
[DOI]
(I p)
Rita A Eckart, Sabine Brantl, Andreas Licht
Search for additional targets of the transcriptional regulator CcpN from Bacillus subtilis.
FEMS Microbiol Lett: 2009, 299(2);223-31
[PubMed:19732150]
[WorldCat.org]
[DOI]
(I p)
Andreas Licht, Sabine Brantl
The transcriptional repressor CcpN from Bacillus subtilis uses different repression mechanisms at different promoters.
J Biol Chem: 2009, 284(44);30032-8
[PubMed:19726675]
[WorldCat.org]
[DOI]
(I p)
Simon Tännler, Eliane Fischer, Dominique Le Coq, Thierry Doan, Emmanuel Jamet, Uwe Sauer, Stéphane Aymerich
CcpN controls central carbon fluxes in Bacillus subtilis.
J Bacteriol: 2008, 190(18);6178-87
[PubMed:18586936]
[WorldCat.org]
[DOI]
(I p)
Andreas Licht, Ralph Golbik, Sabine Brantl
Identification of ligands affecting the activity of the transcriptional repressor CcpN from Bacillus subtilis.
J Mol Biol: 2008, 380(1);17-30
[PubMed:18511073]
[WorldCat.org]
[DOI]
(I p)
Andreas Licht, Sabine Brantl
Transcriptional repressor CcpN from Bacillus subtilis compensates asymmetric contact distribution by cooperative binding.
J Mol Biol: 2006, 364(3);434-48
[PubMed:17011578]
[WorldCat.org]
[DOI]
(P p)
Andreas Licht, Sven Preis, Sabine Brantl
Implication of CcpN in the regulation of a novel untranslated RNA (SR1) in Bacillus subtilis.
Mol Microbiol: 2005, 58(1);189-206
[PubMed:16164558]
[WorldCat.org]
[DOI]
(P p)
Pascale Servant, Dominique Le Coq, Stéphane Aymerich
CcpN (YqzB), a novel regulator for CcpA-independent catabolite repression of Bacillus subtilis gluconeogenic genes.
Mol Microbiol: 2005, 55(5);1435-51
[PubMed:15720552]
[WorldCat.org]
[DOI]
(P p)