Difference between revisions of "BirA"
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|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of biotin synthesis, addition of biotin to proteins | |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of biotin synthesis, addition of biotin to proteins | ||
|- | |- | ||
| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU22440 birA] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 6.769 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 6.769 | ||
| Line 22: | Line 22: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[panB]]'', ''[[cca]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[panB]]'', ''[[cca]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU22440 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU22440 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU22440 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:birA_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:birA_context.gif]] | ||
Revision as of 13:08, 13 May 2013
- Description: trigger enzyme: transcriptional repressor of the biotin operon / biotin-protein ligase
| Gene name | birA |
| Synonyms | |
| Essential | yes PubMed |
| Product | trigger enzyme: transcriptional repressor/ biotin-protein ligase |
| Function | regulation of biotin synthesis, addition of biotin to proteins |
| Gene expression levels in SubtiExpress: birA | |
| MW, pI | 36 kDa, 6.769 |
| Gene length, protein length | 975 bp, 325 aa |
| Immediate neighbours | panB, cca |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biosynthesis of lipids, biosynthesis of cofactors, transcription factors and their control, trigger enzyme, essential genes
This gene is a member of the following regulons
The BirA regulon:
The gene
Basic information
- Locus tag: BSU22440
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: pyrroline-5-carboxylate reductase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P42975
- KEGG entry: [2]
- E.C. number: 6.3.4.15
Additional information
Expression and regulation
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews: PubMed
Original publications
J B Perkins, S Bower, C L Howitt, R R Yocum, J Pero
Identification and characterization of transcripts from the biotin biosynthetic operon of Bacillus subtilis.
J Bacteriol: 1996, 178(21);6361-5
[PubMed:8892842]
[WorldCat.org]
[DOI]
(P p)
S Bower, J B Perkins, R R Yocum, C L Howitt, P Rahaim, J Pero
Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon.
J Bacteriol: 1996, 178(14);4122-30
[PubMed:8763940]
[WorldCat.org]
[DOI]
(P p)
S Bower, J Perkins, R R Yocum, P Serror, A Sorokin, P Rahaim, C L Howitt, N Prasad, S D Ehrlich, J Pero
Cloning and characterization of the Bacillus subtilis birA gene encoding a repressor of the biotin operon.
J Bacteriol: 1995, 177(9);2572-5
[PubMed:7730294]
[WorldCat.org]
[DOI]
(P p)
Operon and expression
Ahmed Gaballa, Gerald L Newton, Haike Antelmann, Derek Parsonage, Heather Upton, Mamta Rawat, Al Claiborne, Robert C Fahey, John D Helmann
Biosynthesis and functions of bacillithiol, a major low-molecular-weight thiol in Bacilli.
Proc Natl Acad Sci U S A: 2010, 107(14);6482-6
[PubMed:20308541]
[WorldCat.org]
[DOI]
(I p)
