Difference between revisions of "NrdF"
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|style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of deoxyribonucleoside triphosphates | |style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of deoxyribonucleoside triphosphates | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU17390 nrdF] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/NrdF NrdF] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/NrdF NrdF] | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nrdE]]'', ''[[ymaB]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[nrdE]]'', ''[[ymaB]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU17390 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU17390 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU17390 Advanced_DNA] |
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|colspan="2" | '''Genetic context''' <br/> [[Image:nrdF_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:nrdF_context.gif]] | ||
Revision as of 12:54, 13 May 2013
- Description: ribonucleoside-diphosphate reductase (major subunit)
| Gene name | nrdF |
| Synonyms | |
| Essential | yes PubMed |
| Product | ribonucleoside-diphosphate reductase (major subunit) |
| Function | synthesis of deoxyribonucleoside triphosphates |
| Gene expression levels in SubtiExpress: nrdF | |
| Interactions involving this protein in SubtInteract: NrdF | |
| Metabolic function and regulation of this protein in SubtiPathways: Purine salvage, Nucleotides (regulation) | |
| MW, pI | 38 kDa, 4.66 |
| Gene length, protein length | 987 bp, 329 aa |
| Immediate neighbours | nrdE, ymaB |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
biosynthesis/ acquisition of nucleotides, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU17390
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin (according to Swiss-Prot)
- Protein family: ribonucleoside diphosphate reductase small chain family (according to Swiss-Prot)
- Paralogous protein(s): YosP
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): Mn(2+) PubMed
- Effectors of protein activity:
Database entries
- UniProt: P50621
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Elisabeth Härtig, Braunschweig, Germany Homepage
Your additional remarks
References
Reviews
Original Publications
Additional publications: PubMed
Elisabeth Härtig, Anja Hartmann, Manuela Schätzle, Alessandra M Albertini, Dieter Jahn
The Bacillus subtilis nrdEF genes, encoding a class Ib ribonucleotide reductase, are essential for aerobic and anaerobic growth.
Appl Environ Microbiol: 2006, 72(8);5260-5
[PubMed:16885274]
[WorldCat.org]
[DOI]
(P p)
C Scotti, A Valbuzzi, M Perego, A Galizzi, A M Albertini
The Bacillus subtilis genes for ribonucleotide reductase are similar to the genes for the second class I NrdE/NrdF enzymes of Enterobacteriaceae.
Microbiology (Reading): 1996, 142 ( Pt 11);2995-3004
[PubMed:8969495]
[WorldCat.org]
[DOI]
(P p)
