Difference between revisions of "PycA"
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|style="background:#ABCDEF;" align="center"|'''Function''' || replenishment of the oxaloacetate pool | |style="background:#ABCDEF;" align="center"|'''Function''' || replenishment of the oxaloacetate pool | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU14860 pycA] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism], [http://subtiwiki.uni-goettingen.de/pathways/biotin/index.html Biotin]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism], [http://subtiwiki.uni-goettingen.de/pathways/biotin/index.html Biotin]''' | ||
| Line 24: | Line 24: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ftsW]]'', ''[[ctaA]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ftsW]]'', ''[[ctaA]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU14860 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU14860 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU14860 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:pycA_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:pycA_context.gif]] | ||
Revision as of 12:44, 13 May 2013
- Description: pyruvate carboxylase
| Gene name | pycA |
| Synonyms | ylaP |
| Essential | no |
| Product | pyruvate carboxylase |
| Function | replenishment of the oxaloacetate pool |
| Gene expression levels in SubtiExpress: pycA | |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism, Biotin | |
| MW, pI | 127 kDa, 5.407 |
| Gene length, protein length | 3444 bp, 1148 aa |
| Immediate neighbours | ftsW, ctaA |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
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Contents
Categories containing this gene/protein
carbon core metabolism, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU14860
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s): biotin
- Effectors of protein activity:
- Localization: membrane associated PubMed
Database entries
- Structure: 3BG5 (S. aureus)
- UniProt: Q9KWU4
- KEGG entry: [3]
- E.C. number: 6.4.1.1
Additional information
PycA binds to StrepTactin, and may be co-purified when purifying Strep-tagged proteins by SPINE.
PycA is subject to Clp-dependent proteolysis upon glucose starvation PubMed
Expression and regulation
- Regulation:
- subject to positive stringent control upon lysine starvation PubMed
- Regulatory mechanism:
- stringent response: due to presence of adenines at +1 and +2 positions of the transcript PubMed
- Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
