Difference between revisions of "NadF"
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|style="background:#ABCDEF;" align="center"|'''Function''' || generation of NADP from NAD | |style="background:#ABCDEF;" align="center"|'''Function''' || generation of NADP from NAD | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11610 nadF] |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.991 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.991 | ||
| Line 21: | Line 21: | ||
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yjbM]]'', ''[[yjbO]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yjbM]]'', ''[[yjbO]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11610 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11610 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11610 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:yjbN_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:yjbN_context.gif]] | ||
Revision as of 12:33, 13 May 2013
- Description: NAD kinase
| Gene name | nadF |
| Synonyms | yjbN, ppnK |
| Essential | yes PubMed |
| Product | inorganic polyphosphate/ATP-NAD kinase |
| Function | generation of NADP from NAD |
| Gene expression levels in SubtiExpress: nadF | |
| MW, pI | 29 kDa, 5.991 |
| Gene length, protein length | 798 bp, 266 aa |
| Immediate neighbours | yjbM, yjbO |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
biosynthesis of cofactors, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU11610
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + NAD+ = ADP + NADP+ (according to Swiss-Prot)
- Protein family: NAD kinase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity: allosterically activated by quinolinic acid
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: O31612
- KEGG entry: [2]
- E.C. number: 2.7.1.23
Additional information
Expression and regulation
- Sigma factor:
- Regulation: expressed during exponential growth
- Regulatory mechanism:
- Additional information:
Biological materials
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Hideaki Nanamiya, Koji Kasai, Akira Nozawa, Choong-Soo Yun, Takakuni Narisawa, Kana Murakami, Yousuke Natori, Fujio Kawamura, Yuzuru Tozawa
Identification and functional analysis of novel (p)ppGpp synthetase genes in Bacillus subtilis.
Mol Microbiol: 2008, 67(2);291-304
[PubMed:18067544]
[WorldCat.org]
[DOI]
(P p)
Silvia Garavaglia, Alessandro Galizzi, Menico Rizzi
Allosteric regulation of Bacillus subtilis NAD kinase by quinolinic acid.
J Bacteriol: 2003, 185(16);4844-50
[PubMed:12897004]
[WorldCat.org]
[DOI]
(P p)
